BD-PM3744-HP-1α(5E3)Mouse Monoclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

HP-1α(5E3)Mouse Monoclonal Antibody

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产品基本信息

产品货号

BD-PM3744

产品名称

HP-1α(5E3)Mouse Monoclonal Antibody

别名

Chromobox protein homolog 5 (Antigen p25) (Heterochromatin protein 1 homolog alpha) (HP1 alpha)

类别

常规抗体

基因名称

CBX5 HP1A

蛋白名称

Chromobox protein homolog 5 (Antigen p25) (Heterochromatin protein 1 homolog alpha) (HP1 alpha)

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

23468

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=23468

Human Swissprot No.

P45973

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P45973/entry

Mouse Swissprot No.

Q61686

Mouse Swissprot Link

http://www.uniprot.org/uniprot/Q61686

免疫原

Recombinant Protein of HP-1α

特异性

The antibody detects endogenous HP-1α protein

稀释度

IF 1:50-200 WB 1:500-2000,IHC-p 1:50-300

预测分子量

22kD

运输及保存条件

-20°C/1 year

宿主

Monoclonal, Mouse

背景介绍

This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008],

组织表达

Epithelium,Fetal brain cortex,Placenta,

细胞定位

Nucleus . Chromosome . Chromosome, centromere . Colocalizes with HNRNPU in the nucleus (PubMed:19617346). Component of centromeric and pericentromeric heterochromatin. Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase. .

功能

function:Component of heterochromatin. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Can interact with lamin B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.,PTM:Phosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle (By similarity). Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.,similarity:Contains 2 chromo domains.,subcellular location:Component of centromeric and pericentromeric heterochromatin. Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase.,subunit:Interacts with SUV420H1 and SUV420H2 (By similarity). Interacts directly with ATRX, CHAF1A, LBR, NIPBL, SP100, STAM2 and TRIM28 via the chromoshadow domain. Can interact directly with CBX3 via the chromoshadow domain. Interacts with histone H3 methylated at 'Lys-9'. Interacts with MIS12 and C20orf127. Interacts with HP1BP3.,

期货

现货

纯化

The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of human-uterus tissue. 1,HP-1α Mouse Monoclonal Antibody（5E3）（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,HP-1α Mouse Monoclonal Antibody（5E3） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,HP-1α Mouse Monoclonal Antibody（5E3） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using HP-1 α Mouse mAb diluted at 1:200

Immunohistochemical analysis of paraffin-embedded Human Placenta Tissue using HP-1 α Mouse mAb diluted at 1:200

Western blot analysis of 1） Hela Cell Lysate, 2）3T3 Cell Lysate, 3） PC12 Cell Lysate using HP-1γα Mouse mAb diluted at 1:1000.