BD-PM3725-TTR(1D7)Mouse Monoclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

TTR(1D7)Mouse Monoclonal Antibody

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产品基本信息

产品货号

BD-PM3725

产品名称

TTR(1D7)Mouse Monoclonal Antibody

别名

Transthyretin (ATTR) (Prealbumin) (TBPA)

类别

常规抗体

基因名称

TTR PALB

蛋白名称

Transthyretin (ATTR) (Prealbumin) (TBPA)

推荐应用

反应种属

Human

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

7276

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=7276

Human Swissprot No.

P02766

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P02766/entry

Mouse Swissprot No.

P07309

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P07309

免疫原

Recombinant Protein of TTR

特异性

The antibody detects endogenous TTR protein

稀释度

IF 1:50-200 WB 1:500-2000,IHC-p 1:50-300

预测分子量

16kD

运输及保存条件

-20°C/1 year

宿主

Monoclonal, Mouse

背景介绍

This gene encodes transthyretin, one of the three prealbumins including alpha-1-antitrypsin, transthyretin and orosomucoid. Transthyretin is a carrier protein; it transports thyroid hormones in the plasma and cerebrospinal fluid, and also transports retinol (vitamin A) in the plasma. The protein consists of a tetramer of identical subunits. More than 80 different mutations in this gene have been reported; most mutations are related to amyloid deposition, affecting predominantly peripheral nerve and/or the heart, and a small portion of the gene mutations is non-amyloidogenic. The diseases caused by mutations include amyloidotic polyneuropathy, euthyroid hyperthyroxinaemia, amyloidotic vitreous opacities, cardiomyopathy, oculoleptomeningeal amyloidosis, meningocerebrovascular amyloidosis, carpal tunnel syndrome, etc. [provided by RefSeq, Jan 2009],

组织表达

Detected in serum and cerebrospinal fluid (at protein level). Highly expressed in choroid plexus epithelial cells. Detected in retina pigment epithelium and liver.

细胞定位

Secreted. Cytoplasm.

功能

disease:Defects in TTR are a cause of hyperthyroxinemia [MIM:176300].,disease:Defects in TTR are the cause of amyloidosis type 1 (AMYL1) [MIM:176300]. AMYL1 is a hereditary generalized amyloidosis due to transthyretin amyloid deposition. Protein fibrils can form in different tissues leading to amyloid polyneuropathies, amyloidotic cardiomyopathy, carpal tunnel syndrome, systemic senile amyloidosis.,disease:Defects in TTR are the cause of amyloidosis type 7 (AMYL7) [MIM:105210]; also known as leptomeningeal amyloidosis or meningocerebrovascular amyloidosis. AMYL7 is a form of hereditary transthyretin amyloidosis characterized by primary involvement of the central nervous system. Neuropathologic examination shows amyloid in the walls of leptomeningeal vessels, in pia arachnoid, and subpial deposits. Some patients also develop vitreous amyloid deposition that leads to visual impairment (oculoleptomeningeal amyloidosis). Clinical features include seizures, stroke-like episodes, dementia, psychomotor deterioration, variable amyloid deposition in the vitreous humor. Mild systemic amyloidosis may occurr.,domain:Each monomer has two 4-stranded beta sheets and the shape of a prolate ellipsoid. Antiparallel beta-sheet interactions link monomers into dimers. A short loop from each monomer forms the main dimer-dimer interaction. These two pairs of loops separate the opposed, convex beta-sheets of the dimers to form an internal channel.,function:Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.,miscellaneous:About 40% of plasma transthyretin circulates in a tight protein-protein complex with the plasma retinol-binding protein (RBP). The formation of the complex with RBP stabilizes the binding of retinol to RBP and decreases the glomerular filtration and renal catabolism of the relatively small RBP molecule. There is evidence for 2 binding sites for RBP, one possibly being a region that includes Ile-104, located on the outer surface of the transthyretin molecule.,miscellaneous:Two binding sites for thyroxine are located in the channel. Less than 1% of plasma prealbumin molecules are normally involved in thyroxine transport. L-thyroxine binds to the transthyretin by an order of magnitude stronger than does the triiodo-L-thyronine. Thyroxine-binding globulin is the major carrier protein for thyroid hormones in man.,online information:Transthyretin entry,similarity:Belongs to the transthyretin family.,subunit:Homotetramer.,tissue specificity:Most abundant in the choroid plexus. Also present in the liver.,

期货

现货

纯化

The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of human-liver-cancer tissue. 1,TTR Mouse Monoclonal Antibody（1D7）（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1,TTR Mouse Monoclonal Antibody（1D7） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Human Liver Carcinoma Tissue using TTR Mouse mAb diluted at 1:200

Immunohistochemical analysis of paraffin-embedded Human Thyroid Tissue using TTR Mouse mAb diluted at 1:200

Western blot analysis of Human Serum using TTR Mouse mAb diluted at 1:2000

Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:200（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.

Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:200（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.