BD-PM3668-CHOP(2B1)Mouse Monoclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

CHOP(2B1)Mouse Monoclonal Antibody

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产品基本信息

产品货号

BD-PM3668

产品名称

CHOP(2B1)Mouse Monoclonal Antibody

别名

DDIT3

类别

常规抗体

基因名称

DDIT3

蛋白名称

DDIT3

推荐应用

反应种属

Human,Rat,Mouse

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

1649

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1649

Human Swissprot No.

P35638

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P35638/entry

Mouse Swissprot No.

P35639

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P35639

Rat Swissprot No.

Q62857

Rat Swissprot Link

http://www.uniprot.org/uniprot/O54941Q62857

免疫原

Synthetic Peptide of CHOP at AA range of 10-90

特异性

CHOP protein detects endogenous levels of DDIT3

稀释度

WB 1:1000-2000, IHC 1:100-200 IF 1:200

预测分子量

27kD

运输及保存条件

-20°C/1 year

宿主

Monoclonal, Mouse

背景介绍

This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. [provided by RefSeq, Aug 2010],

组织表达

Muscle,Skeletal muscle,

细胞定位

Cytoplasm . Nucleus . Present in the cytoplasm under non-stressed conditions and ER stress leads to its nuclear accumulation. .

信号通路

MAPK_ERK_Growth;MAPK_G_Protein;

功能

disease:A chromosomal aberration involving DDIT3 is found in a form of malignant myxoid liposarcoma [MIM:126337]. Translocation t(12;16)(q13;p11) with FUS.,function:Inhibits the DNA-binding activity of C/EBP and LAP by forming heterodimers that cannot bind DNA.,similarity:Belongs to the bZIP family.,similarity:Contains 1 bZIP domain.,subunit:Heterodimer.,

期货

现货

纯化

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Immunohistochemical analysis of paraffin-embedded Human-stomach tissue. 1,CHOP Mouse Monoclonal Antibody（2B1） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunofluorescence analysis of Mouse-brain tissue. 1,CHOP Mouse Monoclonal Antibody（2B1）（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunohistochemical analysis of paraffin-embedded Human Stomach Carcinoma Tissue using CHOP Mouse mAb diluted at 1:200.

Immunohistochemical analysis of paraffin-embedded Human Pancreas Carcinoma Tissue using CHOP Mouse mAb diluted at 1:200.

Western blot analysis of Mouse Liver Tissue Lysate using CHOP Mouse mAb diluted at 1:2000.