产品名称
CKAP2 Rabbit Polyclonal Antibody
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/26586
Human Swissprot No.
Q8WWK9
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q8WWK9/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/80986
Mouse Swissprot No.
Q3V1H1
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q3V1H1
免疫原
Synthesized peptide derived from human CKAP2 AA range: 298-348
特异性
This antibody detects endogenous levels of CKAP2 at Human/Mouse
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a cytoskeleton-associated protein that stabalizes microtubules and plays a role in the regulation of cell division. The encoded protein is itself regulated through phosphorylation at multiple serine and threonine residues. There is a pseudogene of this gene on chromosome 14. Alternative splicing results in multiple transcript variations. [provided by RefSeq, Nov 2013],
组织表达
Abundant in testis, thymus, and in tumor derived cell lines, while barely detectable in liver, prostate, and kidney.
细胞定位
Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Contrary to the ectopically expressed protein, endogenous CKAP2 does not colocalize with microtubules in G1, S and early G2. At late G2 and prophase after separation of duplicated centrosomes, colocalizes with gamma-tubulin and centrosome-proximal microtubules. From prometaphase through anaphase B, colocalizes with mitotic spindle poles and spindle microtubules. During cytokinesis, absent from midbody microtubules.
功能
developmental stage:Present at the G1/S boundary. Accumulates as cells progress from S to G2 into mitosis. Rapidly degraded during mitosis exit by CDH1-activated anaphase promoting complex/cyclosome (APC/C).,function:Possesses microtubule stabilizing properties. Involved in regulating aneuploidy, cell cycling, and cell death in a p53/TP53-dependent manner.,induction:Up-regulated in primary human gastric cancers.,sequence caution:Contaminating sequence. Potential poly-A sequence.,similarity:Belongs to the CKAP2 family.,subcellular location:Contrary to the ectopically expressed protein, endogenous CKAP2 does not colocalize with microtubules in G1, S and early G2. At late G2 and prophase after separation of duplicated centrosomes, colocalizes with gamma-tubulin and centrosome-proximal microtubules. From prometaphase through anaphase B, colocalizes with mitotic spindle poles and spindle microtubules. During cytokinesis, absent from midbody microtubules.,subunit:Associates with alpha- and beta-tubulins.,tissue specificity:Abundant in testis, thymus, and in tumor derived cell lines, while barely detectable in liver, prostate, and kidney.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
