BD-PM3649-LC3A(5G10)Mouse Monoclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

LC3A(5G10)Mouse Monoclonal Antibody

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产品基本信息

产品货号

BD-PM3649

产品名称

LC3A(5G10)Mouse Monoclonal Antibody

别名

Microtubule-associated proteins 1A/1B light chain 3A (Autophagy-related protein LC3 A) (Autophagy-related ubiquitin-like modifier LC3 A) (MAP1 light chain 3-like protein 1) (MAP1A/MAP1B light chain 3 A) (MAP1A/MAP1B LC3 A) (Microtubule-associated protein 1 light chain 3 alpha)

类别

常规抗体

基因名称

MAP1LC3A

蛋白名称

LC3A

推荐应用

反应种属

Human,Rat,Mouse

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

84557

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=84557

Human Swissprot No.

Q9H492

Human Swissprot Link

http://www.uniprot.org/uniprotkb/Q9H492/entry

Mouse Swissprot No.

Q91VR7

Mouse Swissprot Link

http://www.uniprot.org/uniprot/Q91VR7

Rat Swissprot No.

Q6XVN8

Rat Swissprot Link

http://www.uniprot.org/uniprot/O54941Q6XVN8

免疫原

Synthetic Peptide of LC3A

特异性

LC3A protein detects endogenous levels of LC3A

稀释度

WB 1:1000-2000, IHC 1:100-200 IF 1:200

预测分子量

14,16kD

运输及保存条件

-20°C/1 year

宿主

Monoclonal, Mouse

背景介绍

MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis. [provided by RefSeq, Feb 2012],

组织表达

Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.

细胞定位

Cytoplasmic vesicle, autophagosome membrane ; Lipid-anchor . Endomembrane system ; Lipid-anchor . Cytoplasm, cytoskeleton . LC3-II binds to the autophagic membranes. .

功能

function:Probably involved in formation of autophagosomal vacuoles (autophagosomes).,PTM:The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.,similarity:Belongs to the MAP1 LC3 family.,subcellular location:LC3-II binds to the autophagic membranes.,subunit:3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins.,tissue specificity:Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.,

期货

现货

纯化

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,LC3A Mouse Monoclonal Antibody（5G10） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,LC3A Mouse Monoclonal Antibody（5G10） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunofluorescence analysis of Human-lung-cancer tissue. 1,LC3A Mouse Monoclonal Antibody（5G10）（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of Mouse-spleen tissue. 1,LC3A Mouse Monoclonal Antibody（5G10）（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunohistochemical analysis of paraffin-embedded Human Heptacarcinoma Tissue using LC3A Mouse mAb diluted at 1:200.

Immunohistochemical analysis of paraffin-embedded Human Prostate Carcinoma Tissue using LC3A Mouse mAb diluted at 1:200.

Western blot analysis of 1） Hela Cell Lysate, 2） 3T3 Cell Lysate, 3） Rat Brain Tissue Lysate using LC3A Mouse mAb diluted at 1:1000.