产品名称
VP33B Rabbit Polyclonal Antibody
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/26276
Human Swissprot No.
Q9H267
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q9H267/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/233405
Mouse Swissprot No.
P59016
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/P59016
Rat Gene Link
https://www.uniprot.org/uniprot/64060
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/Q63616
免疫原
Synthesized peptide derived from human VP33B AA range: 12-62
特异性
This antibody detects endogenous levels of VP33B at Human/Mouse/Rat
宿主
Polyclonal, Rabbit,IgG
背景介绍
Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene is a member of the Sec-1 domain family, and encodes the human ortholog of rat Vps33b which is homologous to the yeast class C Vps33 protein. The mammalian class C vacuolar protein sorting proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. Mutations in this gene are associated with arthrogryposis-renal dysfunction-cholestasis syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014],
组织表达
Ubiquitous; highly expressed in testis and low expression in the lung.
细胞定位
Late endosome membrane ; Peripheral membrane protein; Cytoplasmic side. Lysosome membrane ; Peripheral membrane protein; Cytoplasmic side. Early endosome . Cytoplasmic vesicle, clathrin-coated vesicle . Recycling endosome . Colocalizes in clusters with VIPAS39 at cytoplasmic organelles (PubMed:19109425). Colocalizes with RAB11A and VIPAS39 on recycling endosomes (PubMed:22753090). Colocalizes with AP-3, clathrin, Rab5 and Rab7b (PubMed:21411634). Colocalizes with M.tuberculosis PtpA in the cytosol of tuberculosis-infected macrophages and associates with phagosomes (PubMed:18474358). .
功能
disease:Defects in VPS33B are the cause of arthrogryposis-renal dysfunction-cholestasis syndrome (ARC) [MIM:208085]. ARC is an autosomal recessive multisystem disorder, characterized by neurogenic arthrogryposis multiplex congenita, renal tubular dysfunction and neonatal cholestasis with bile duct hypoplasia and low gamma glutamyl transpeptidase activity. Platelet dysfunction is common.,function:May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.,similarity:Belongs to the STXBP/unc-18/SEC1 family.,subcellular location:Cytoplasmic, peripheral membrane protein associated with late endosomes/lysosomes.,tissue specificity:Ubiquitous; highly expressed in testis and low expression in the lung.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
