产品名称
GM130 Rabbit Polyclonal Antibody
别名
Golgin subfamily A member 2 (130 kDa cis-Golgi matrix protein;GM130;GM130 autoantigen;Golgin-95)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Swissprot No.
Q08379
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q08379/entry
免疫原
Synthesized peptide derived from human GM130
特异性
This antibody detects endogenous levels of human GM130
稀释度
WB 1:500 - 1:2000. ELISA: 1:10000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This encoded protein has been postulated to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. [provided by RefSeq, Feb 2010],
组织表达
Brain,Cervix,Colon adenocarcinoma,Epithelium,Heart,Hepatocyte,Liver,Placenta,Thymus,
细胞定位
Golgi apparatus, cis-Golgi network membrane ; Peripheral membrane protein ; Cytoplasmic side . Endoplasmic reticulum-Golgi intermediate compartment membrane ; Peripheral membrane protein ; Cytoplasmic side . Cytoplasm, cytoskeleton, spindle pole . Associates with the mitotic spindle during mitosis (PubMed:26165940). .
功能
domain:Extended rod-like protein with coiled-coil domains.,function:Golgi auto-antigen; probably involved in maintaining cis-Golgi structure.,sequence caution:Sequence differs from that shown after position 814 due to an internal deletion.,similarity:Belongs to the GOLGA2 family.,subunit:Part of a larger oligomeric complex. Interacts with p115 (By similarity). Interacts with RAB1B that has been activated by GTP-binding. Interacts with GORASP1/GRASP65 and ZFPL1.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
