产品名称
eIF5A/eIF5A2 Rabbit Polyclonal Antibody
别名
Eukaryotic translation initiation factor 5A-1 (eIF-5A-1;eIF-5A1;Eukaryotic initiation factor 5A isoform 1;eIF-5A;Rev-binding factor;eIF-4D)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Swissprot No.
P63241/Q9GZV4
Human Swissprot Link
https://www.uniprot.org/uniprotkb/P63241/entry
免疫原
Synthesized peptide derived from human eIF5A/eIF5A2
特异性
This antibody detects endogenous levels of human eIF5A/eIF5A2
稀释度
WB 1:500-2000, ELISA(peptide)1:5000-20000
宿主
Polyclonal, Rabbit,IgG
组织表达
Expressed in umbilical vein endothelial cells and several cancer cell lines (at protein level).
细胞定位
Cytoplasm . Nucleus . Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side . Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions (PubMed:19379712, PubMed:27306458). Nuclear export of hypusinated protein is mediated by XPO4 (PubMed:10944119, PubMed:27306458). .
功能
function:The precise role of eIF-5A in protein biosynthesis is not known but it functions by promoting the formation of the first peptide bond.,PTM:eIF-5A seems to be the only eukaryotic protein to have an hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).,similarity:Belongs to the eIF-5A family.,subunit:Found in a complex with Ran and XPO4. The hypusine modification increases the interaction with XPO4.,tissue specificity:Expressed in umbilical vein endothelial cells and several cancer cell lines (at protein level).,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
