产品名称
LC3B(9H5)Mouse Monoclonal Antibody
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=81631
Human Swissprot No.
Q9GZQ8
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q9GZQ8/entry
Mouse Swissprot No.
Q9CQV6
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9CQV6
免疫原
Recombinant Protein of LC3B of MAP1LC3B
特异性
LC3B protein detects endogenous levels of MAP1LC3B
稀释度
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000. IF 1:50-200
背景介绍
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008],
组织表达
Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver.
细胞定位
Cytoplasmic vesicle, autophagosome membrane ; Lipid-anchor . Endomembrane system ; Lipid-anchor . Mitochondrion membrane ; Lipid-anchor . Cytoplasm, cytoskeleton . Cytoplasmic vesicle . LC3-II binds to the autophagic membranes. LC3-II localizes with the mitochondrial inner membrane during Parkin-mediated mitophagy (PubMed:28017329). Localizes also to discrete punctae along the ciliary axoneme. .
功能
caution:PubMed:12740394 has shown that the protein is cleaved at Lys-122 but PubMed:15355958 has shown that the cleavage site is at Gly-120 as in other mammalian orthologs.,function:Probably involved in formation of autophagosomal vacuoles (autophagosomes).,PTM:The precursor molecule is cleaved by APG4B/ATG4B to form LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form LC3-II.,similarity:Belongs to the MAP1 LC3 family.,subcellular location:LC3-II binds to the autophagic membranes.,subunit:3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins.,tissue specificity:Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver.,
纯化
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
