BD-PM3636-Ubiquitin(5F1)Mouse Monoclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Ubiquitin(5F1)Mouse Monoclonal Antibody

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产品基本信息

产品货号

BD-PM3636

产品名称

Ubiquitin(5F1)Mouse Monoclonal Antibody

类别

常规抗体

蛋白名称

Ubiquitin

推荐应用

反应种属

Human,Rat,Mouse,Pig

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

7314

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=7314

Human Swissprot No.

PAN

Human Swissprot Link

https://www.uniprot.org/uniprotkb/PAN/entry

免疫原

Synthetic Peptide of Ubiquitin

特异性

Ubiquitin protein detects endogenous levels of Ubiquitin

稀释度

WB 1:1000-2000, IHC 1:100-200 IF 1:200

运输及保存条件

-20°C/1 year

宿主

Monoclonal, Mouse

背景介绍

UBB (ubiquitin B) encodes ubiquitin, one of the most conserved proteins known. Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. UBB consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. An aberrant form of this protein has been detected in patients with Alzheimer's disease and Down syndrome. Pseudogenes of UBB are located on chromosomes 1, 2, 13, and 17. Alternative splicing results in multiple transcript variants.

期货

现货

纯化

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1,Ubiquitin Mouse Monoclonal Antibody（5F1） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,Ubiquitin Mouse Monoclonal Antibody（5F1） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunofluorescence analysis of Human-stomach-cancer tissue. 1,Ubiquitin Mouse Monoclonal Antibody（5F1）（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of Mouse-brain tissue. 1,Ubiquitin Mouse Monoclonal Antibody（5F1）（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of Rat-brain tissue. 1,Ubiquitin Mouse Monoclonal Antibody（5F1）（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using Ubiquitin Mouse mAb diluted at 1:200.

Immunohistochemical analysis of paraffin-embedded Human Stomach Carcinoma Tissue using Ubiquitin Mouse mAb diluted at 1:200.

Western blot analysis of 1） Hela Cell Lysate, 2） 3T3 Cell Lysate, 3） Rat Brain Tissue Lysate using Ubiquitin Mouse mAb diluted at 1:1000.