BD-PM3626-CystatinC(7F11)Mouse Monoclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

CystatinC(7F11)Mouse Monoclonal Antibody

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产品基本信息

产品货号

BD-PM3626

产品名称

CystatinC(7F11)Mouse Monoclonal Antibody

别名

CST3

类别

常规抗体

基因名称

CST3

蛋白名称

CST3

推荐应用

反应种属

Human

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

1471

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1471

Human Swissprot No.

P01034

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P01034/entry

Mouse Swissprot No.

P21460

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P21460

免疫原

Recombinant Protein of Cystatin C of CST3

特异性

Cystatin C protein detects endogenous levels of CST3

稀释度

IF 1:50-200 WB 1:1000-2000, IHC 1:100-200

预测分子量

14kD

运输及保存条件

-20°C/1 year

宿主

Monoclonal, Mouse

背景介绍

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes the most abundant extracellular inhibitor of cysteine proteases, which is found in high concentrations in biological fluids and is expressed in virtually all organs of the body. A mutation in this gene has been associate

组织表达

Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland.

细胞定位

Secreted .

功能

disease:Defects in CST3 are the cause of amyloidosis type 6 (AMYL6) [MIM:105150]; also known as hereditary cerebral hemorrhage with amyloidosis (HCHWA), cerebral amyloid angiopathy (CAA) or cerebroarterial amyloidosis Icelandic type. AMYL6 is a hereditary generalized amyloidosis due to cystatin C amyloid deposition. Cystatin C amyloid accumulates in the walls of arteries, arterioles, and sometimes capillaries and veins of the brain, and in various organs including lymphoid tissue, spleen, salivary glands, and seminal vesicles. Amyloid deposition in the cerebral vessels results in cerebral amyloid angiopathy, cerebral hemorrage and premature stroke. Cystatin C levels in the cerebrospinal fluid are abnormally low.,disease:Genetic variations in CST3 are associated with age-related macular degeneration type 11 (ARMD11) [MIM:611953]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.,function:As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.,miscellaneous:Potential cerebrospinal fluid marker for the diagnosis of Creutzfeldt-Jakob disease.,similarity:Belongs to the cystatin family.,subunit:Homodimer.,tissue specificity:Found in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland.,

期货

现货

纯化

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Immunofluorescence analysis of human-liver-cancer tissue. 1,Cystatin C Mouse Monoclonal Antibody（7F11）（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,Cystatin C Mouse Monoclonal Antibody（7F11） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Human Kidney Tissue using Cystatin C Mouse mAb diluted at 1:200

Immunohistochemical analysis of paraffin-embedded Human Brain Tissue using Cystatin C Mouse mAb diluted at 1:200.

Western Blot analysis of Cystatin C protein using antibody diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded Human Fallopian tube. 1, Antibody was diluted at 1:400（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.

Immunohistochemical analysis of paraffin-embedded Human heart. 1, Antibody was diluted at 1:100（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.