产品名称
GPX4 Rabbit Polyclonal Antibody
别名
Phospholipid hydroperoxide glutathione peroxidase, mitochondrial (PHGPx) (EC 1.11.1.12) (Glutathione peroxidase 4) (GPx-4) (GSHPx-4)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.05% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/2879
Human Swissprot No.
P36969
Human Swissprot Link
https://www.uniprot.org/uniprotkb/P36969/entry
免疫原
Synthesized peptide derived from human GPX4 AA range: 84-134
特异性
This antibody detects endogenous levels of GPX4 at Human
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of hydrogen peroxide, organic hydroperoxides and lipid hydroperoxides, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme has a high preference for lipid hydroperoxides and protects cells against membrane lipid peroxidation and cell death. It is also required for normal sperm development; thus, it has been identified as a 'moonlighting' protein because of its ability to serve dual functions as a peroxidase, as well as a structural protein in mature spermatozoa. Mutations in this gene are associated with Sedaghatian type of spondylometaphyseal dysplasia (SMDS). This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2016],
组织表达
Present primarily in testis. Expressed in platelets (at protein level) (PubMed:11115402).
细胞定位
[Isoform Mitochondrial]: Mitochondrion .; [Isoform Cytoplasmic]: Cytoplasm .
功能
catalytic activity:2 glutathione + a lipid hydroperoxide = glutathione disulfide + lipid + 2 H(2)O.,disease:Defects in GPX4 may be a cause of infertility.,function:Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage.,similarity:Belongs to the glutathione peroxidase family.,subunit:Monomer. Has a tendency to form higher mass oligomers.,tissue specificity:Present primarily in testis.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
