产品名称
a-actinin(4A12)Mouse Monoclonal Antibody
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=87
Human Swissprot No.
P12814
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P12814/entry
Mouse Swissprot No.
Q7TPR4
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q7TPR4
Rat Swissprot Link
http://www.uniprot.org/uniprot/O54941Q9Z1P2
免疫原
Recombinant Protein of a-actinin of ACTN1
特异性
a-actinin Monoclonal Antibody(4A12) detects endogenous levels of ACTN1
稀释度
IHC-p 1:100-500;IF ICC 1:100-500,WB 1:500-2000
背景介绍
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
组织表达
Colon,Epithelium,Hepatocyte,Neuroblastoma,Placenta,Platelet,Skin,Tumor of tongue,
细胞定位
Cytoplasm, cytoskeleton . Cytoplasm, myofibril, sarcomere, Z line . Cell membrane . Cell junction . Cell projection, ruffle . Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle. Colocalizes with PSD in membrane ruffles and central reticular structures. .
信号通路
Focal adhesion;Adherens_Junction;Adherens_Junction;Leukocyte transendothelial migration;Regulates Actin and Cytoskeleton;Systemic lupus erythematosus;Arrhythmogenic right ventricular cardiomyopathy (ARVC);
功能
function:F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.,similarity:Belongs to the alpha-actinin family.,similarity:Contains 1 actin-binding domain.,similarity:Contains 2 CH (calponin-homology) domains.,similarity:Contains 2 EF-hand domains.,similarity:Contains 4 spectrin repeats.,subcellular location:Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle.,subunit:Homodimer; antiparallel. Interacts with DDN, MYOZ2, PDLIM2, TTID and LPP.,
纯化
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
