产品名称
NEDD4L Rabbit Polyclonal Antibody
别名
E3 ubiquitin-protein ligase NEDD4-like (EC 6.3.2.-) (NEDD4.2) (Nedd4-2)
基因名称
NEDD4L KIAA0439 NEDL3
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.08% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/23327
Human Swissprot No.
Q96PU5
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q96PU5/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/0
Mouse Swissprot No.
Q8CFI0
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q8CFI0
免疫原
Synthesized peptide derived from human NEDD4L AA range: 28-78
特异性
This antibody detects endogenous levels of NEDD4L at Human/Mouse
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein mediates the ubiquitination of multiple target substrates and plays a critical role in epithelial sodium transport by regulating the cell surface expression of the epithelial sodium channel, ENaC. Single nucleotide polymorphisms in this gene may be associated with essential hypertension. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012],
组织表达
Ubiquitously expressed, with highest levels in prostate, pancreas, and kidney (PubMed:14615060, PubMed:15496141, PubMed:19664597). Expressed in melanocytes (PubMed:23999003).
细胞定位
Cytoplasm . Golgi apparatus . Endosome, multivesicular body . May be recruited to exosomes by NDFIP1.
功能
function:E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFR1 ubiquitination and proteasome-dependent degradation. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. Promotes ubiquitination and degradation of SGK.,induction:By androgens in prostate, and by albumin in kidney.,pathway:Protein modification; protein ubiquitination.,PTM:Phosphorylated by SGK or PKA; which impairs interaction with SCNN. Interaction with YWHAH inhibits dephosphorylation.,similarity:Contains 1 C2 domain.,similarity:Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.,similarity:Contains 4 WW domains.,subunit:Interacts with NDFIP2 and UBE2E3 (By similarity). Interacts via its WW domains with SCNN1A, SCNN1B, SCNN1G, SCN1A, SCN2A, SCN3A, SCN5A, SCN8A, SCN9A, SCN10A and CLCN5. Interacts with SMAD2, SMAD3, SMAD6 and SMAD7. The phosphorylated form interacts with 14-3-3 proteins. Interacts with Epstein-Barr virus LMP2A. Interacts with NDFIP1 in vitro.,tissue specificity:Ubiquitously expressed, with highest levels in prostate, pancreas and kidney.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
