产品名称
HIF-1 β/ARNT(4C5)Mouse Monoclonal Antibody
别名
ARNT; BHLHE2; Aryl hydrocarbon receptor nuclear translocator; ARNT protein; Class E basic helix-loop-helix protein 2; bHLHe2; Dioxin receptor, nuclear translocator; Hypoxia-inducible factor 1-beta; HIF-1-beta; HIF1-beta
蛋白名称
Aryl hydrocarbon receptor nuclear translocator (ARNT protein) (Class E basic helix-loop-helix protein 2) (bHLHe2) (Dioxin receptor, nuclear translocator) (Hypoxia-inducible factor 1-beta) (HIF-1-beta) (HIF1-beta)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=405
Human Swissprot No.
P27540
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P27540/entry
Mouse Swissprot No.
P53762
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P53762
免疫原
Recombinant Protein of HIF-1 β
特异性
HIF-1 β/ARNT protein detects endogenous levels of HIF-1 β/ARNT
稀释度
WB 1:1000-2000, IHC 1:100-200. IF 1:50-200
背景介绍
This gene encodes a protein containing a basic helix-loop-helix domain and two characteristic PAS domains along with a PAC domain. The encoded protein binds to ligand-bound aryl hydrocarbon receptor and aids in the movement of this complex to the nucleus, where it promotes the expression of genes involved in xenobiotic metabolism. This protein is also a co-factor for transcriptional regulation by hypoxia-inducible factor 1. Chromosomal translocation of this locus with the ETV6 (ets variant 6) gene on chromosome 12 have been described in leukemias. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2013],
组织表达
Aorta endothelial cell,Brain,Kidney,Thalamus,Uterus,
信号通路
Pathways in cancer;Renal cell carcinoma;
功能
function:Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,similarity:Contains 1 PAC (PAS-associated C-terminal) domain.,similarity:Contains 2 PAS (PER-ARNT-SIM) domains.,subunit:Efficient DNA binding requires dimerization with another bHLH protein. Forms a heterodimer with AHR, AHRR, HIF1A and EPAS1/HIF2A as well as with other bHLH proteins. Interacts with TACC3 (By similarity). Interacts with NOCA7.,
纯化
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
