产品名称
GLI-1 Rabbit Polyclonal Antibody
别名
Zinc finger protein GLI1 (Glioma-associated oncogene) (Oncogene GLI)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=2735
Human Swissprot No.
P08151
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P08151/entry
Mouse Swissprot No.
P47806
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P47806
免疫原
Synthesized peptide derived from human GLI-1. at AA range: 460-490
特异性
This antibody detects endogenous levels of GLI-1
稀释度
WB 1:500-2000, ELISA 1:10000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009],
组织表达
Detected in testis (at protein level) (PubMed:2105456). Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract (PubMed:19878745). Isoform 1 is detected in brain, spleen, pancreas, liver, kidney and placenta; isoform 2 is not detectable in these tissues (PubMed:19706761).
细胞定位
Cytoplasm . Nucleus . Tethered in the cytoplasm by binding to SUFU (PubMed:10806483). Activation and translocation to the nucleus is promoted by interaction with STK36 (PubMed:10806483). Phosphorylation by ULK3 may promote nuclear localization (PubMed:19878745). Translocation to the nucleus is promoted by interaction with ZIC1 (PubMed:11238441). .; [Isoform 2]: Cytoplasm . Nucleus .
信号通路
Hedgehog;Pathways in cancer;Basal cell carcinoma;
功能
disease:Defects in GLI1 may be a cause of breast cancer.,function:May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling and thus cell proliferation and differentiation.,induction:Amplified in glioblastoma cells.,similarity:Belongs to the GLI C2H2-type zinc-finger protein family.,similarity:Contains 5 C2H2-type zinc fingers.,subcellular location:Tethered in the cytoplasm by binding to SUFU. Activation and translocation to the nucleus is promoted by interaction with STK36.,tissue specificity:Testis, myometrium and fallopian tube.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
