产品名称
DAAM1 Rabbit Polyclonal Antibody
别名
Disheveled-associated activator of morphogenesis 1
蛋白名称
Disheveled-associated activator of morphogenesis 1
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=23002
Human Swissprot No.
Q9Y4D1
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q9Y4D1/entry
Mouse Swissprot No.
Q8BPM0
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q8BPM0
免疫原
Synthetic peptide from human protein at AA range: 400-500
特异性
The antibody detects endogenous DAAM1
稀释度
WB 1:500-2000, ELISA 1:10000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
Cell motility, adhesion, cytokinesis, and other functions of the cell cortex are mediated by reorganization of the actin cytoskeleton and several formin homology (FH) proteins have been associated with these processes. The protein encoded by this gene contains two FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. A key regulator of cytoskeletal architecture, the small GTPase Rho, is activated during development by Wnt/Fz signaling to control cell polarity and movement. The protein encoded by this gene is thought to function as a scaffolding protein for the Wnt-induced assembly of a disheveled (Dvl)-Rho complex. This protein also promotes the nucleation and elongation of new actin filaments and regulates cell growth through the stabilization of microtubules. Alternative splicing results in multiple transcript variants encoding distinct
组织表达
Expressed in all tissues examined.
细胞定位
Cytoplasm . Cytoplasm, cytoskeleton, cilium basal body . Perinuclear.
功能
domain:The C-terminal DAD domain may participate in intramolecular interactions with the N-terminus.,function:Binds to disheveled (Dvl) and Rho, and mediates Wnt-induced Dvl-Rho complex formation. May play a role as a scaffolding protein to recruit Rho-GDP and Rho-GEF, thereby enhancing Rho-GTP formation. Can direct nucleation and elongation of new actin filaments.,similarity:Belongs to the formin homology family.,similarity:Contains 1 DAD (diaphanous autoregulatory) domain.,similarity:Contains 1 FH1 (formin homology 1) domain.,similarity:Contains 1 FH2 (formin homology 2) domain.,similarity:Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.,subcellular location:Perinuclear.,subunit:Homodimer. Interacts with CIP4, FNBP1 and FNBP1L. Interacts with the SH3 domains of Abl, BTK, endophilin, spectrin and SRC. Binds specifically to GTP-bound CDC42 and RHOA.,tissue specificity:Expressed in all tissues examined.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
