产品名称
GADD34 Rabbit Polyclonal Antibody
别名
Protein phosphatase 1 regulatory subunit 15A (Growth arrest and DNA damage-inducible protein GADD34) (Myeloid differentiation primary response protein MyD116 homolog)
蛋白名称
Protein phosphatase 1 regulatory subunit 15A (Growth arrest and DNA damage-inducible protein GADD34) (Myeloid differentiation primary response protein MyD116 homolog)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=23645
Human Swissprot No.
O75807
Human Swissprot Link
http://www.uniprot.org/uniprotkb/O75807/entry
Mouse Swissprot No.
P17564
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P17564
免疫原
Synthetic peptide from human protein at AA range: 430-490
特异性
The antibody detects endogenous GADD34
稀释度
WB 1:500-2000, ELISA 1:10000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The induction of this gene by ionizing radiation occurs in certain cell lines regardless of p53 status, and its protein response is correlated with apoptosis following ionizing radiation. [provided by RefSeq, Jul 2008],
细胞定位
Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side . Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side . Associates with membranes via an N-terminal amphipathic intramembrane region. .
功能
function:Recruits the serine/threonine-protein phosphatase PP1 to dephosphorylate the translation initiation factor eIF-2A/EIF2S1, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. Down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 by PP1. May promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'.,induction:By methyl methanesulfonate and ionizing irradiation. By IL24 in melanoma cells; which induces apoptosis.,miscellaneous:The phosphatase activity of the PPP1R15A-PP1 complex toward EIF2S1 is specifically inhibited by Salubrinal, a drug that protects cells from endoplasmic reticulum stress.,PTM:Phosphorylated on tyrosine by LYN; which impairs its antiproliferative activity.,similarity:Belongs to the PPP1R15 family.,subunit:Interacts with PCNA (By similarity). Interacts with LYN and MLL. Interacts with PP1, PPP1R1A and SMARCB1. Interacts with SMAD7. Interacts with BAG1.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
