产品名称
TRIM27 Rabbit Polyclonal Antibody
别名
Zinc finger protein RFP (EC 6.3.2.-) (RING finger protein 76) (Ret finger protein) (Tripartite motif-containing protein 27)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.10% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/5987
Human Swissprot No.
P14373
Human Swissprot Link
https://www.uniprot.org/uniprotkb/P14373/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/19720
Mouse Swissprot No.
Q62158
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q62158
免疫原
Synthesized peptide derived from human TRIM27 AA range: 34-84
特异性
This antibody detects endogenous levels of TRIM27 at Human/Mouse
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nuclear matrix. It interacts with the enhancer of polycomb protein and represses gene transcription. It is also thought to be involved in the differentiation of male germ cells. Fusion of the N-terminus of this protein with the truncated C-terminus of the RET gene product has been shown to result in production of the ret transforming protein. [provided by RefSeq, Jul 2008],
组织表达
Expressed in testis namely within the seminiferous tubules.
细胞定位
Nucleus . Cytoplasm . Nucleus, PML body . Early endosome . Nuclear or cytoplasmic depending on the cell type (By similarity). Colocalized with PML and EIF3S6 in nuclear bodies. Recruited to retromer-containing endosomes via interaction with MAGEL2 (PubMed:23452853). .
功能
disease:A chromosomal aberration involving TRIM27 is a cause of thyroid papillary carcinoma (PACT) [MIM:188550]. Translocation t(6;10)(p21.3;q11.2) with RET. The translocation generates RFP/RET and delta RFP/RET oncogenes.,domain:The coiled-coil region mediates interaction with EPC1 and CHD4. The B box and coiled-coil domains mediate interaction with PML. The B box and the distal coiled-coil domains mediate homomultimerisation. The B30.2 domain mediates interaction with EIF3S6.,function:Has a transcriptional repressor activity by cooperating with EPC1. Induces apoptosis by activating Jun N-terminal kinase and p38 kinase and also increases caspase-3-like activity independently of mitochondrial events. May function in male germ cell development. Has DNA-binding activity and preferentially bound to double-stranded DNA.,similarity:Belongs to the TRIM/RBCC family.,similarity:Contains 1 B box-type zinc finger.,similarity:Contains 1 B30.2/SPRY domain.,similarity:Contains 1 RING-type zinc finger.,subcellular location:Nuclear or cytoplasmic depending on the cell type (By similarity). Colocalized with PML and EIF3S6 in nuclear bodies.,subunit:Homomultimerizes. Interacts with PML, EIF3S6, EPC1, CHD4 and EID1.,tissue specificity:Expressed in testis namely within the seminiferous tubules.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
