产品名称
Cdk6 Rabbit Polyclonal Antibody
别名
Cyclin-dependent kinase 6 (EC 2.7.11.22;Cell division protein kinase 6;Serine/threonine-protein kinase PLSTIRE)
蛋白名称
Cyclin-dependent kinase 6 (EC 2.7.11.22) (Cell division protein kinase 6) (Serine/threonine-protein kinase PLSTIRE)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1021
Human Swissprot No.
Q00534
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q00534/entry
Mouse Swissprot No.
Q64261
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q64261
免疫原
Synthetic peptide from human protein at AA range: 280-325
特异性
The antibody detects endogenous Cdk6
稀释度
WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
cyclin dependent kinase 6(CDK6) Homo sapiens The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Expression of this gene is up-regulated in some types of cancer. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefS
组织表达
Expressed ubiquitously. Accumulates in squamous cell carcinomas, proliferating hematopoietic progenitor cells, beta-cells of pancreatic islets of Langerhans, and neuroblastomas. Reduced levels in differentiating cells.
细胞定位
Cytoplasm. Nucleus. Cell projection, ruffle. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Localized to the ruffling edge of spreading fibroblasts. Kinase activity only in nucleus. Localized to the cytosol of neurons and showed prominent staining around either side of the nucleus (By similarity). Present in the cytosol and in the nucleus in interphase cells and at the centrosome during mitosis from prophase to telophase (PubMed:23918663). .
信号通路
Cell_Cycle_G1S;Cell_Cycle_G2M_DNA;p53;Pathways in cancer;Pancreatic cancer;Glioma;Melanoma;Chronic myeloid leukemia;Small cell lung cancer;Non-small cell lung cancer;
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Probably involved in the control of the cell cycle. Interacts with D-type G1 cyclins.,polymorphism:Genetic variations in CDK6 influences stature as a quantitative trait type 11 (STQTL11) [MIM:612223]. Adult height is an easily observable and highly heritable complex continuous trait. Because of this, it is a model trait for studying genetic influence on quantitative traits.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.,similarity:Contains 1 protein kinase domain.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
