BD-PT5880-EGF Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

EGF Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT5880

产品名称

EGF Rabbit Polyclonal Antibody

别名

Pro-epidermal growth factor (EGF) [Cleaved into: Epidermal growth factor (Urogastrone)]

类别

常规抗体

基因名称

EGF

蛋白名称

Pro-epidermal growth factor (EGF) [Cleaved into: Epidermal growth factor (Urogastrone)]

推荐应用

反应种属

Human,Mouse

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

1950

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1950

Human Swissprot No.

P01133

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P01133/entry

Mouse Gene ID

13645

Mouse Swissprot No.

P01132

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P01132

免疫原

Synthetic peptide from human protein at AA range: 1000-1060

特异性

The antibody detects endogenous EGF

稀释度

WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000. IF 1:50-200

预测分子量

130kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016],

组织表达

Expressed in kidney, salivary gland, cerebrum and prostate.

细胞定位

Membrane; Single-pass type I membrane protein.

信号通路

MAPK_ERK_Growth;MAPK_G_Protein;ErbB_HER;Cytokine-cytokine receptor interaction;Endocytosis;Focal adhesion;Gap junction;Regulates Actin and Cytoskeleton;Pathways in cancer;Pancreatic cancer;Endometrial cancer;Glioma;Prostate cancer;Melanoma;Bladder cancer;

功能

disease:Defects in EGF are the cause of hypomagnesemia type 4 (HOMG4) [MIM:611718]; also known as renal hypomagnesemia normocalciuric. HOMG4 is a disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion. Clinical features include seizures, mild-to mederate psychomotor retardation, and brisk tendon reflexes.,function:EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6.,online information:Epidermal growth factor entry,similarity:Contains 9 EGF-like domains.,similarity:Contains 9 LDL-receptor class B repeats.,tissue specificity:Expressed in kidney, salivary gland, cerebrum and prostate.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western blot analysis of 293T mouse-kidney lysate, antibody was diluted at 2000. Secondary antibody was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded human-kidney-cancer, antibody was diluted at 1:200

Immunohistochemical analysis of paraffin-embedded human-stomach-cancer, antibody was diluted at 1:200

Immunohistochemical analysis of paraffin-embedded Human skin. 1, Antibody was diluted at 1:200（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.

Immunohistochemical analysis of paraffin-embedded Human gastric cancer. 1, Antibody was diluted at 1:100（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.