BD-PM3519-α-tubulin(Acetyl Lys40)(4A8)Mouse Monoclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

α-tubulin(Acetyl Lys40)(4A8)Mouse Monoclonal Antibody

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产品基本信息

产品货号

BD-PM3519

产品名称

α-tubulin(Acetyl Lys40)(4A8)Mouse Monoclonal Antibody

别名

Tubulin alpha-1B chain (Alpha-tubulin ubiquitous) (Tubulin K-alpha-1) (Tubulin alpha-ubiquitous chain)

类别

常规抗体

基因名称

TUBA1B

蛋白名称

Tubulin alpha-1B chain (Alpha-tubulin ubiquitous) (Tubulin K-alpha-1) (Tubulin alpha-ubiquitous chain)

推荐应用

反应种属

Human,Rat,Mouse,Drosophila

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

7277

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=7277

Human Swissprot No.

P68363

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P68363/entry

Mouse Swissprot No.

P05213

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P05213

Rat Swissprot No.

Q6P9V9

Rat Swissprot Link

http://www.uniprot.org/uniprot/O54941Q6P9V9

免疫原

Synthetic Peptide of α-tubulin (Acetyl Lys40)

特异性

α-tubulin (Acetyl Lys40) protein detects endogenous levels of α-tubulin (Acetyl Lys40)

稀释度

WB 1:1000-2000, IHC 1:50-100 IF 1:200

预测分子量

52kD

运输及保存条件

-20°C/1 year

宿主

Monoclonal, Mouse

背景介绍

function:Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.,PTM:Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.,similarity:Belongs to the tubulin family.,subunit:Dimer of alpha and beta chains.,

组织表达

Brain,Cajal-Retzius cell,Colon,Embryonic kidney,Eye,Fetal brain cortex,Keratinocyte

细胞定位

Cytoplasm, cytoskeleton.

信号通路

Gap junction;Pathogenic Escherichia coli infection;

功能

期货

现货

纯化

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,α-tubulin （Acetyl Lys40） Monoclonal Antibody（4A8） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1,α-tubulin （Acetyl Lys40） Monoclonal Antibody（4A8） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1,α-tubulin （Acetyl Lys40） Monoclonal Antibody（4A8） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunofluorescence analysis of Human-liver-cancer tissue. 1,α-tubulin （Acetyl Lys40） Monoclonal Antibody（4A8）（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of Mouse-lung tissue. 1,α-tubulin （Acetyl Lys40） Monoclonal Antibody（4A8）（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of Rat-spinal-cord tissue. 1,α-tubulin （Acetyl Lys40） Monoclonal Antibody（4A8）（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Western blot analysis of extracts from Hela cells, untreated （-） or treated with TSA （1μM, 18 hr; +）, using Acetyl- a-tubulin（Lys40） Mouse mAb 1:2000.

Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma using a-tubulin（Acetyl Lys40） Mouse mAb diluted at 1:200.

Immunohistochemical analysis of paraffin-embedded Mouse Brain Tissue using a-tubulin（Acetyl Lys40） Mouse mAb diluted at 1:200.