产品名称
GRP78 BiP Rabbit Polyclonal Antibody
别名
78 kDa glucose-regulated protein (GRP-78) (Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78) (Heat shock 70 kDa protein 5) (Immunoglobulin heavy chain-binding protein) (BiP)
反应种属
Human,Mouse,Rat,Fish
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=3309
Human Swissprot No.
P11021
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P11021/entry
Mouse Swissprot No.
P20029
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P20029
Rat Swissprot Link
http://www.uniprot.org/uniprot/O54941P06761
免疫原
Synthetic peptide from human protein at AA range: 505-570
特异性
The antibody detects endogenous GRP78 BiP
稀释度
WB 1:500 - 1:2000.IHC-p:f:1:50-300. ELISA 1:10000-20000. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.[provided by RefSeq, Sep 2010],
组织表达
Articular cartilage,Brain,Cajal-Retzius cell,Cervix carcino
细胞定位
Endoplasmic reticulum lumen . Melanosome . Cytoplasm . Cell surface . Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:12643545). Localizes to the cell surface of epithelial cells in response to high levels of free iron (PubMed:20484814, PubMed:24355926, PubMed:27159390). .
信号通路
Antigen processing and presentation;Prion diseases;
功能
disease:Autoantigen in rheumatoid arthritis [MIM:180300].,function:Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER.,similarity:Belongs to the heat shock protein 70 family.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,subunit:Interacts with DNAJC1 (via J domain) (By similarity). Component of an EIF2 complex at least composed of CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Part a large chaperone multiprotein complex comprising CABP1, DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with TMEM132A.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
