BD-PT5815-MIB1 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

MIB1 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT5815

产品名称

MIB1 Rabbit Polyclonal Antibody

别名

MIB1 DIP1 KIAA1323 ZZANK2

类别

常规抗体

基因名称

MIB1 DIP1 KIAA1323 ZZANK2

蛋白名称

mindbomb homolog 1 (Drosophila)

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

57534

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=57534

Human Swissprot No.

Q86YT6

Human Swissprot Link

http://www.uniprot.org/uniprotkb/Q86YT6/entry

Mouse Gene ID

225164

Mouse Swissprot No.

Q80SY4

Mouse Swissprot Link

http://www.uniprot.org/uniprot/Q80SY4

免疫原

Synthetic peptide from human protein at AA range: 901-950

特异性

The antibody detects endogenous MIB1 protein

稀释度

IHC-p: 100-300.WB 1:500-2000, ELISA 1:10000-20000. IF 1:50-200

预测分子量

130kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

This gene encodes a protein containing multiple ankyrin repeats and RING finger domains that functions as an E3 ubiquitin ligase. The encoded protein positively regulates Notch signaling by ubiquitinating the Notch receptors, thereby facilitating their endocytosis. This protein may also promote the ubiquitination and degradation of death-associated protein kinase 1 (DAPK1). [provided by RefSeq, Jun 2013],

组织表达

Widely expressed at low level. Expressed at higher level in spinal cord, ovary, whole brain, and all specific brain regions examined.

细胞定位

Cytoplasm . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriolar satellite . Cell membrane . Localizes to the plasma membrane (By similarity). According to PubMed:15048887, it is mitochondrial, however such localization remains unclear. Displaced from centriolar satellites in response to cellular stress, such as ultraviolet light (UV) radiation or heat shock. .

功能

function:E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis.,miscellaneous:In epilepsy brain tissue, levels of expression are increased in the cytoplasm and microsomal fractions (endoplasmic reticulum).,pathway:Protein modification; protein ubiquitination.,PTM:Ubiquitinated. Possibly via autoubiquitination.,similarity:Contains 1 ZZ-type zinc finger.,similarity:Contains 2 MIB/HERC2 domains.,similarity:Contains 3 RING-type zinc fingers.,similarity:Contains 9 ANK repeats.,subcellular location:Localizes to the plasma membrane (By similarity). According to PubMed:15048887, it is mitochondrial, however such localization remains unclear.,tissue specificity:Widely expressed at low level. Expressed at higher level in spinal cord, ovary, whole brain, and all specific brain regions examined.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western Blot analysis of 293t cells using Antibody diluted at 1000. Secondary antibody was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200

Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:200

Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:200（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.

Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.