产品名称
TyrRS Rabbit Polyclonal Antibody
别名
Tyrosine--tRNA ligase, cytoplasmic (EC 6.1.1.1) (Tyrosyl-tRNA synthetase) (TyrRS)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=8565
Human Swissprot No.
P54577
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P54577/entry
Mouse Swissprot No.
Q91WQ3
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q91WQ3
Rat Swissprot Link
http://www.uniprot.org/uniprot/O54941Q4KM49
免疫原
The antiserum was produced against synthesized peptide derived from the C-terminal region of human YARS. AA range:451-500
特异性
TyrRS Polyclonal Antibody detects endogenous levels of TyrRS
稀释度
WB 1:500-2000,IHC 1:50-300 ELISA 1:10000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Tyrosyl-tRNA synthetase belongs to the class I tRNA synthetase family. Cytokine activities have also been observed for the human tyrosyl-tRNA synthetase, after it is split into two parts, an N-terminal fragment that harbors the catalytic site and a C-terminal fragment found only in the mammalian enzyme. The N-terminal fragment is an interleukin-8-like cytokine, whereas the released C-terminal fragment is an EMAP II-like cytokine. [provided by RefSeq, Jul 2008],
组织表达
B-cell lymphoma,Bone,Lung,Platelet,
信号通路
Aminoacyl-tRNA biosynthesis;
功能
catalytic activity:ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr).,disease:Defects in YARS are the cause of Charcot-Marie-Tooth disease dominant intermediate type C (CMTDIC) [MIM:608323]. CMTDIC is a form of Charcot-Marie-Tooth disease characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec.,function:Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).,similarity:Belongs to the class-I aminoacyl-tRNA synthetase family.,similarity:Contains 1 tRNA-binding domain.,subunit:Homodimer.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
