BD-PT5745-E-Selectin Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

E-Selectin Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT5745

产品名称

E-Selectin Rabbit Polyclonal Antibody

别名

selectin E

类别

常规抗体

基因名称

SELE ELAM1

蛋白名称

E-Selectin

推荐应用

反应种属

Human,Rat,Mouse

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

6401

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=6401

Human Swissprot No.

P16581

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P16581/entry

Mouse Gene ID

20339

Mouse Swissprot No.

Q00690

Mouse Swissprot Link

http://www.uniprot.org/uniprot/Q00690

免疫原

The antiserum was produced against synthesized peptide derived from the N-terminal region of human SELE. AA range:100-150

特异性

E-Selectin Polyclonal Antibody detects endogenous levels of E-Selectin

稀释度

IHC-p: 100-300.WB 1:500-2000, ELISA 1:10000-20000. IF 1:50-200

预测分子量

66kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

The protein encoded by this gene is found in cytokine-stimulated endothelial cells and is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. It exhibits structural features such as the presence of lectin- and EGF-like domains followed by short consensus repeat (SCR) domains that contain 6 conserved cysteine residues. These proteins are part of the selectin family of cell adhesion molecules. Adhesion molecules participate in the interaction between leukocytes and the endothelium and appear to be involved in the pathogenesis of atherosclerosis. [provided by RefSeq, Jul 2008],

组织表达

Umbilical vein endothelial cell,

细胞定位

Cell membrane ; Single-pass type I membrane protein.

信号通路

Cell adhesion molecules (CAMs);

功能

function:Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis.,online information:E-selectin,polymorphism:A polymorphism in position 149 is associated with a higher risk of coronary artery disease (CAD). A significantly higher mutation frequency (Arg-149) is observed in patients with angiographically proven severe atherosclerosis compared with an unselected population (Ser-149).,similarity:Belongs to the selectin/LECAM family.,similarity:Contains 1 C-type lectin domain.,similarity:Contains 1 EGF-like domain.,similarity:Contains 6 Sushi (CCP/SCR) domains.,subunit:Interacts with PSGL1/SELPLG through the sialyl Lewis X epitope. PSGL1 sulfation appears not to be required for this interaction.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western Blot analysis of K562 SH-SY5Y mouse-brain cells using E-Selectin Polyclonal Antibody diluted at 1:500. Secondary antibody was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:200

Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200

Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:200（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.

Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.