产品名称
MAFA Rabbit Polyclonal Antibody
别名
Transcription factor MafA (Pancreatic beta-cell-specific transcriptional activator) (Transcription factor RIPE3b1) (V-maf musculoaponeurotic fibrosarcoma oncogene homolog A)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.36% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/389692
Human Swissprot No.
Q8NHW3
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q8NHW3/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/378435
Mouse Swissprot No.
Q8CF90
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q8CF90
免疫原
Synthesized peptide derived from human MAFA AA range: 204-254
特异性
This antibody detects endogenous levels of MAFA at Human/Mouse
宿主
Polyclonal, Rabbit,IgG
背景介绍
MAFA is a transcription factor that binds RIPE3b, a conserved enhancer element that regulates pancreatic beta cell-specific expression of the insulin gene (INS; MIM 176730) (Olbrot et al., 2002 [PubMed 12011435]).[supplied by OMIM, Mar 2008],
组织表达
Expressed in the islets of Langerhans (at protein level).
功能
function:Acts as a transcriptional factor. Specifically binds the insulin enhancer element RIPE3b and activates insulin gene expression. Cooperates synergistically with NEUROD1 and PDX1. Phosphorylation by GSK3 increases its transcriptional activity and is required for its oncogenic activity. Involved either as an oncogene or as a tumor suppressor, depending on the cell context.,induction:Up-regulated by glucose.,PTM:Ser-14 and Ser-65 appear to be the major phosphorylation sites. Phosphorylated by MAPK13 on serine and threonine residues (Probable). Phosphorylation by GSK3 requires prior phosphorylation of Ser-65 by another kinase. Phosphorylation proceeds then from Ser-61 to Thr-57, Thr-53 and Ser-49. GSK3-mediated phosphorylation increases its transcriptional activity through the recruitment of the coactivator PCAF, is required for its transforming activity and leads to its degradation through an ubiquitin/proteasome-dependent pathway.,PTM:Ubiquitinated, leading to its degradation by the proteasome.,similarity:Belongs to the bZIP family. Maf subfamily.,similarity:Contains 1 bZIP domain.,subcellular location:Detected in nuclei of pancreas islet beta cells.,subunit:Binds DNA as a homodimer (Probable). Interacts with NEUROD1, PCAF and PDX1.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
