BD-PM3430-MICU1(Mix)Mouse Monoclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

MICU1(Mix)Mouse Monoclonal Antibody

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产品基本信息

产品货号

BD-PM3430

产品名称

MICU1(Mix)Mouse Monoclonal Antibody

别名

Calcium uptake protein 1, mitochondrial; Atopy-related autoantigen CALC; ara CALC; Calcium-binding atopy-related autoantigen 1; allergen Hom s 4

类别

常规抗体

基因名称

MICU1

蛋白名称

Calcium uptake protein 1, mitochondrial

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS, pH 7.4, containing 0.5%BSA, 0.02% New type preservative N as Preservative and 50% Glycerol.

Human Gene ID

10367

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=10367

Human Swissprot No.

Q9BPX6

Human Swissprot Link

http://www.uniprot.org/uniprotkb/Q9BPX6/entry

Mouse Swissprot No.

Q8VCX5

Mouse Swissprot Link

http://www.uniprot.org/uniprot/Q8VCX5

Rat Swissprot No.

Q6P6Q9

Rat Swissprot Link

http://www.uniprot.org/uniprot/O54941Q6P6Q9

免疫原

Recombinant Protein of MICU1

特异性

The antibody detects endogenous MICU1 protein.

稀释度

WB 1:1000-2000 IHC 1:100-200 IF 1:200

预测分子量

55kD

运输及保存条件

-20°C/1 year

宿主

Monoclonal, Mouse

背景介绍

This gene encodes an essential regulator of mitochondrial Ca2+ uptake under basal conditions. The encoded protein interacts with the mitochondrial calcium uniporter, a mitochondrial inner membrane Ca2+ channel, and is essential in preventing mitochondrial Ca2+ overload, which can cause excessive production of reactive oxygen species and cell stress. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Mar 2013],

组织表达

Expressed in epithelial cell lines. Strongly expressed in epidermal keratinocytes and dermal endothelial cells.

细胞定位

Mitochondrion inner membrane ; Single-pass membrane protein . Mitochondrion intermembrane space .

功能

allergen:Causes an allergic reaction in human. Binds to IgE from atopic dermatitis (AD) patients. Identified as an IgE autoantigen in atopic dermatitis (AD) patients with severe skin manifestations.,function:Induces T helper 1-mediated autoreactivity, which is accompanied by the release of IFNG.,sequence caution:Contaminating sequence. Sequence of unknown origin in the N-terminal part.,similarity:Contains 2 EF-hand domains.,tissue specificity:Expressed in epithelial cell line. Strongly expressed in epidermal keratinocytes and dermal endothelial cells.,

期货

现货

纯化

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.

Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,MICU1 Monoclonal Antibody（Mix） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-spinal-cord tissue. 1,MICU1 Monoclonal Antibody（Mix） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1,MICU1 Monoclonal Antibody（Mix） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunofluorescence analysis of Human-appendix tissue. 1,MICU1 Monoclonal Antibody（Mix）（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Western blot analysis of 1） MCF7, 2） Mouse Brain Tissue, 3） Rat Brain Tissue using MICU1 Monoclonal Antibody.

Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma using MICU1 Monoclonal Antibody.