BD-PM3419-Survivin(Mix)Mouse Monoclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Survivin(Mix)Mouse Monoclonal Antibody

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产品基本信息

产品货号

BD-PM3419

产品名称

Survivin(Mix)Mouse Monoclonal Antibody

别名

BIRC5; API4; IAP4; Baculoviral IAP repeat-containing protein 5; Apoptosis inhibitor 4; Apoptosis inhibitor survivin

类别

常规抗体

基因名称

BIRC5

蛋白名称

Baculoviral IAP repeat-containing protein 5

推荐应用

反应种属

Human,Rat

存储缓冲液

PBS, pH 7.4, containing 0.5%BSA, 0.02% New type preservative N as Preservative and 50% Glycerol.

Human Gene ID

332

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=332

Human Swissprot No.

O15392

Human Swissprot Link

http://www.uniprot.org/uniprotkb/O15392/entry

Mouse Swissprot No.

O70201

Mouse Swissprot Link

http://www.uniprot.org/uniprot/O70201

Rat Swissprot No.

Q9JHY7

Rat Swissprot Link

http://www.uniprot.org/uniprot/O54941Q9JHY7

免疫原

Recombinant Protein of Survivin

特异性

The antibody detects endogenous Survivin protein.

稀释度

WB 1:1000-2000 IHC 1:200-500. IF 1:50-200

预测分子量

16kD

运输及保存条件

-20°C/1 year

宿主

Monoclonal, Mouse

背景介绍

This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors, yet low in adult tissues. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jun 2011],

组织表达

Expressed only in fetal kidney and liver, and to lesser extent, lung and brain (PubMed:10626797). Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas (PubMed:14741722, PubMed:16329164). Also expressed in various renal cell carcinoma cell lines (PubMed:10626797). Expressed in cochlea including the organ of Corti, the lateral wall, the interdental cells of the Limbus as well as in Schwann cells and cells of the cochlear nerve and the spiral ganglions (at protein level). Not expressed in cells of the inner and outer sulcus or the Reissner's membrane (at protein level) (PubMed:21364656, PubMed:20627126).

细胞定位

Cytoplasm . Nucleus . Chromosome . Chromosome, centromere . Cytoplasm, cytoskeleton, spindle . Chromosome, centromere, kinetochore . Midbody . Localizes at the centromeres from prophase to metaphase, at the spindle midzone during anaphase and a the midbody during telophase and cytokinesis. Accumulates in the nucleus upon treatment with leptomycin B (LMB), a XPO1/CRM1 nuclear export inhibitor (By similarity). Localizes on chromosome arms and inner centromeres from prophase through metaphase. Localizes to kinetochores in metaphase, distributes to the midzone microtubules in anaphase and at telophase, localizes exclusively to the midbody (PubMed:11084331). Colocalizes with AURKB at mitotic chromosomes (PubMed:14610074). Acetylation at Lys-129 directs its localization to the nucleus by enhancing homodimerization and thereby inhibiting XPO1/CRM1-mediated nuclear export (PubMed:20826784). .

信号通路

Pathways in cancer;Colorectal cancer;

功能

domain:The BIR repeat is necessary and sufficient for HBXIP binding.,function:May play a role in neoplasia. May counteract a default induction of apoptosis in G2/M phase. Interacts with tubulin. Inhibitor of caspase-3 and caspase-7. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Isoforms 2 and 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.,similarity:Belongs to the IAP family.,similarity:Contains 1 BIR repeat.,subcellular location:Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes.,subunit:Homodimer. When phosphorylated, interacts with HBXIP; the resulting complex binds pro-caspase-9, as well as active caspase-9, but much less efficiently. Component of the CPC at least composed of BIRC5/survivin, CDCA8/borealin, INCENP and AURKB/Aurora-B. Interacts with EVI5.,tissue specificity:Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines.,

期货

现货

纯化

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.

Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1,Survivin Monoclonal Antibody（Mix） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-spinal-cord tissue. 1,Survivin Monoclonal Antibody（Mix） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue. 1,Survivin Monoclonal Antibody（Mix） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Western blot analysis of 1） Hela, 2） 293, 3） PC12 using Survivin Monoclonal Antibody.

Immunohistochemical analysis of paraffin-embedded human colon caricnoma using Survivin Monoclonal Antibody.

Western blot detection of Survivin in human breast cancer cell line MCF-7（A） and Cal51 （B） using Survivin mouse mAb （1:1000 diluted）.Predicted band size: 16kDa.Observed band size:16kDa. Picture was kindly provided by our customer from Tianjin Medical University Cancer Institute and Hospital

Immunohistochemical analysis of paraffin-embedded Human breast cancer. 1, Using Survivin Mouse mAb diluted at 1:200 （4°,overnight）. 2, High-pressure and temperature Citric acid, pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 50min）. Picture was kindly provided by our customer from Tianjin Medical University Cancer Institute and Hospital

Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.