产品名称
Topo IIIα Rabbit Polyclonal Antibody
别名
TOP3A; TOP3; DNA topoisomerase 3-alpha; DNA topoisomerase III alpha
蛋白名称
DNA topoisomerase 3-alpha
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=7156
Human Swissprot No.
Q13472
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q13472/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=21975
Mouse Swissprot No.
O70157
Mouse Swissprot Link
http://www.uniprot.org/uniprot/O70157
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=
Rat Swissprot Link
http://www.uniprot.org/uniprot/
免疫原
Synthesized peptide derived from Topo IIIα . at AA range: 350-430
特异性
Topo IIIα Polyclonal Antibody detects endogenous levels of Topo IIIα protein.
稀释度
WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus reducing the number of supercoils and altering the topology of DNA. This enzyme forms a complex with BLM which functions in the regulation of recombination in somatic cells. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016],
组织表达
High expression is found in testis, heart, skeletal muscle and pancreas.
细胞定位
Mitochondrion matrix .
信号通路
Homologous recombination;
功能
catalytic activity:ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.,function:Reduces the number of supercoils in a highly negatively supercoiled DNA. Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells.,similarity:Belongs to the prokaryotic type I/III topoisomerase family.,subunit:Directly interacts with BLM and RMI1. Component of the RMI complex, containing at least TOP3A, RMI1 and RMI2. The RMI complex interacts with BLM.,tissue specificity:High expression is found in testis, heart, skeletal muscle and pancreas.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
