BD-PM3402-CYCS(4B10)Mouse Monoclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

CYCS(4B10)Mouse Monoclonal Antibody

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产品基本信息

产品货号

BD-PM3402

产品名称

CYCS(4B10)Mouse Monoclonal Antibody

别名

CYCS; CYC; Cytochrome c

类别

常规抗体

基因名称

CYCS

蛋白名称

Cytochrome c

推荐应用

反应种属

Human,Mouse,Rat,Chicken

存储缓冲液

PBS, pH 7.4, containing 0.5%BSA, 0.02% New type preservative N as Preservative and 50% Glycerol.

Human Gene ID

54205

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=54205

Human Swissprot No.

P99999

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P99999/entry

Mouse Swissprot No.

P62897

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P62897

Rat Swissprot No.

P62898

Rat Swissprot Link

http://www.uniprot.org/uniprot/O54941P62898

免疫原

Recombinant Protein of CYCS

特异性

The antibody detects endogenous CYCS protein.

稀释度

WB 1:1000-5000 IHC 1:500-1000 IF 1:200

预测分子量

14kD

运输及保存条件

-20°C/1 year

宿主

Monoclonal, Mouse

背景介绍

This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.[provided by RefSeq, Jul 2010],

组织表达

Amygdala,Bone marrow,Brain,Embryo,Heart,Kidney,Lung,Skeletal muscle,Skin,Testis,Uri

细胞定位

Mitochondrion intermembrane space. Loosely associated with the inner membrane.

信号通路

p53;Apoptosis_Inhibition;Apoptosis_Mitochondrial;Apoptosis_Overview;Alzheimer's disease;Parkinson's disease;Amyotrophic lateral sclerosis (ALS);Huntington's disease;Pathways in cancer;Colorectal cancer;Small cell lung cancer;Viral myocarditis;

功能

disease:Defects in CYCS are the cause of thrombocytopenia type 4 (THC4) [MIM:612004]; also known as autosomal dominant thrombocytopenia type 4. Thrombocytopenia is the presence of relatively few platelets in blood. THC4 is a non-syndromic form of thrombocytopenia. Clinical manifestations of thrombocytopenia are absent or mild. THC4 may be caused by dysregulated platelet formation.,function:Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.,function:Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.,online information:Life shuttle - Issue 76 of November 2006,PTM:Binds 1 heme group per subunit.,similarity:Belongs to the cytochrome c family.,

期货

现货

纯化

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.

Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1,CYCS Monoclonal Antibody（4B10） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,CYCS Monoclonal Antibody（4B10） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,CYCS Monoclonal Antibody（4B10） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunofluorescence analysis of Human-liver-cancer tissue. 1,CYCS Monoclonal Antibody（4B10）（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of Mouse-spleen tissue. 1,CYCS Monoclonal Antibody（4B10）（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of Rat-liver tissue. 1,CYCS Monoclonal Antibody（4B10）（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Western blot analysis of 1） Hela, 2） 293T, 3） 3T3, 4） Mouse Liver Tissue, 5） Rat Liver Tissue, 6） Rat Kidney Tissue using CYCS Monoclonal Antibody.

Immunohistochemical analysis of paraffin-embedded human Breast caricnoma using CYCS Monoclonal Antibody.

Western Blot analysis of chicken cell lysis using Antibody diluted at 1:1000