BD-PM3378-HAO1(Mix)Mouse Monoclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

HAO1(Mix)Mouse Monoclonal Antibody

产品详情相关文献产品问答相关产品

产品基本信息

产品货号

BD-PM3378

产品名称

HAO1(Mix)Mouse Monoclonal Antibody

别名

Hydroxyacid oxidase 1; HAOX1; Glycolate oxidase; GOX

类别

常规抗体

基因名称

HAO1

蛋白名称

Hydroxyacid oxidase 1

推荐应用

反应种属

Mouse,Rat

存储缓冲液

PBS, pH 7.4, containing 0.5%BSA, 0.02% New type preservative N as Preservative and 50% Glycerol.

Human Gene ID

54363

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=54363

Human Swissprot No.

Q9UJM8

Human Swissprot Link

http://www.uniprot.org/uniprotkb/Q9UJM8/entry

Mouse Swissprot No.

Q9WU19

Mouse Swissprot Link

http://www.uniprot.org/uniprot/Q9WU19

免疫原

Recombinant Protein of HAO1

特异性

The antibody detects endogenous HAO1 protein.

稀释度

WB 1:1000-2000 IF 1:200 IHC 1:50-300

预测分子量

41kD

运输及保存条件

-20°C/1 year

宿主

Monoclonal, Mouse

背景介绍

This gene is one of three related genes that have 2-hydroxyacid oxidase activity yet differ in encoded protein amino acid sequence, tissue expression and substrate preference. Subcellular location of the encoded protein is the peroxisome. Specifically, this gene is expressed primarily in liver and pancreas and the encoded protein is most active on glycolate, a two-carbon substrate. The protein is also active on 2-hydroxy fatty acids. The transcript detected at high levels in pancreas may represent an alternatively spliced form or the use of a multiple near-consensus upstream polyadenylation site. [provided by RefSeq, Jul 2008],

组织表达

Highly expressed in liver.

细胞定位

Peroxisome matrix .

信号通路

Glyoxylate and dicarboxylate metabolism;

功能

catalytic activity:(S)-2-hydroxy acid + O(2) = 2-oxo acid + H(2)O(2).,cofactor:FMN.,function:Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids.,pathway:Organic acid metabolism; glycolic acid degradation; 3-phospho-D-glyceric acid from glycolic acid: step 1/4.,similarity:Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.,similarity:Contains 1 FMN hydroxy acid dehydrogenase domain.,tissue specificity:Liver.,

期货

现货

纯化

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.

Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,HAO1 Monoclonal Antibody（Mix） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-liver tissue. 1,HAO1 Monoclonal Antibody（Mix） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1,HAO1 Monoclonal Antibody（Mix） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunofluorescence analysis of Human-appendix tissue. 1,HAO1 Monoclonal Antibody（Mix）（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of Mouse-spleen tissue. 1,HAO1 Monoclonal Antibody（Mix）（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Western blot analysis of 1） Mouse Liver Tissue, 2） Rat Liver Tissue using HAO1 Monoclonal Antibody.