BD-PT5580-CD19 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

CD19 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT5580

产品名称

CD19 Rabbit Polyclonal Antibody

别名

CD19; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Differentiation antigen CD19; T-cell surface antigen Leu-12; CD19

类别

常规抗体

基因名称

CD19

蛋白名称

B-lymphocyte antigen CD19

推荐应用

反应种属

Human,Rat,Mouse

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

930

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=930

Human Swissprot No.

P15391

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P15391/entry

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=

Mouse Swissprot No.

P25918

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P25918

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=

Rat Swissprot Link

http://www.uniprot.org/uniprot/

免疫原

Synthesized peptide derived from B-lymphocyte antigen CD19 at AA range: 191-240

特异性

CD19 Polyclonal Antibody detects endogenous levels of CD19 protein.

稀释度

WB 1:500-2000;Flow Cyt 1:50-200;IHC-p 1:100-500;IF(paraffin section);ELISA 1:5000-20000

预测分子量

61kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [provided by RefSeq, Jul 2008],

组织表达

Detected on marginal zone and germinal center B cells in lymph nodes (PubMed:2463100). Detected on blood B cells (at protein level) (PubMed:2463100, PubMed:16672701).

细胞定位

Cell membrane ; Single-pass type I membrane protein . Membrane raft ; Single-pass type I membrane protein .

信号通路

Hematopoietic cell lineage;B_Cell_Antigen;Primary immunodeficiency;

功能

disease:Defects in CD19 are a cause of hypogammaglobulinemia [MIM:107265].,function:Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.,online information:CD19 mutation db,PTM:Phosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation.,similarity:Contains 2 Ig-like C2-type (immunoglobulin-like) domains.,subunit:Forms a complex with CD21, CD81 and CD225 in the membrane of mature B cells. Interacts with VAV. Interacts with GRB2 and SOS when phosphorylated on Tyr-348 and/or Tyr-378. Interacts with PLCG2 when phosphorylated on Tyr-409.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western Blot analysis of mouse brain cells using CD19 Polyclonal Antibody. Antibody was diluted at 1:2000. Secondary antibody was diluted at 1:20000

Western Blot analysis of MOUSE-BRAIN cells using CD19 Polyclonal Antibody diluted at 1：2000. Secondary antibody was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded human-tonsils, antibody was diluted at 1:100

Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100

Western Blot analysis of mouse-kidney mouse-spleen using CD19 Polyclonal Antibody diluted at 1:1500. Secondary antibody was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:200（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.

Immunohistochemical analysis of paraffin-embedded Human liver. 1, Antibody was diluted at 1:100（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.