BD-PT5553-M-CSF Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

M-CSF Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT5553

产品名称

M-CSF Rabbit Polyclonal Antibody

别名

CSF1; Macrophage colony-stimulating factor 1; CSF-1; M-CSF; MCSF; Lanimostim

类别

常规抗体

基因名称

CSF1

蛋白名称

Macrophage colony-stimulating factor 1

推荐应用

反应种属

Human,Rat,Mouse

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

1435

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1435

Human Swissprot No.

P09603

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P09603/entry

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=

Mouse Swissprot No.

P07141

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P07141

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=

Rat Swissprot Link

http://www.uniprot.org/uniprot/

免疫原

The antiserum was produced against synthesized peptide derived from the C-terminal region of human CSF1. AA range:505-554

特异性

M-CSF Polyclonal Antibody detects endogenous levels of M-CSF protein.

稀释度

WB 1:500 - 1:2000. IHC-p: 1:100-1:300. ELISA: 1:10000.. IF 1:50-200

预测分子量

48kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011],

组织表达

Endometrium,Kidney,Pancreatic carcinoma,T lymphoblast,Trophoblast,Urine,

细胞定位

Cell membrane ; Single-pass type I membrane protein .; [Processed macrophage colony-stimulating factor 1]: Secreted, extracellular space.

信号通路

Cytokine-cytokine receptor interaction;Hematopoietic cell lineage;

功能

function:Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy.,PTM:Glycosylation and proteolytic cleavage yield different soluble forms. A high molecular weight soluble form is a proteoglycan containing chondroitin sulfate.,PTM:Isoform 1 is N- and O-glycosylated. Isoform 3 is N-glycosylated.,subunit:Homodimer or heterodimer; disulfide-linked.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western Blot analysis of NIH-3T3, Hela cells using M-CSF Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Western Blot analysis of 3T3 cells using M-CSF Polyclonal Antibody. Secondary antibody was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded human-tonsils, antibody was diluted at 1:100

Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:100

Western blot analysis of various cell Lysate, antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:200（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.

Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.