产品名称
FGF-2 Rabbit Polyclonal Antibody
别名
FGF2; FGFB; Fibroblast growth factor 2; FGF-2; Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2
蛋白名称
Fibroblast growth factor 2
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=2247
Human Swissprot No.
P09038
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P09038/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=14173
Mouse Swissprot No.
P15655
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P15655
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=54250
Rat Swissprot Link
http://www.uniprot.org/uniprot/P13109
免疫原
The antiserum was produced against synthesized peptide derived from the Internal region of human FGF2. AA range:151-200
特异性
FGF-2 Polyclonal Antibody detects endogenous levels of FGF-2 protein.
稀释度
WB 1:500 - 1:2000. IHC-p: 1:100-1:300. ELISA: 1:10000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq, Jul 2008],
组织表达
Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.
细胞定位
Secreted . Nucleus . Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane (PubMed:20230531). Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol (PubMed:22321063). Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57 (PubMed:22321063). .
信号通路
MAPK_ERK_Growth;MAPK_G_Protein;Regulates Actin and Cytoskeleton;Pathways in cancer;Melanoma;
功能
function:The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.,miscellaneous:This protein binds heparin more strongly than does aFGF.,PTM:Several N-termini starting at positions 48, 54, 47 and 65 have been identified by direct sequencing.,sequence caution:Unusual initiator. The initiator methionine is coded by a non-canonical CTG leucine codon.,similarity:Belongs to the heparin-binding growth factors family.,subunit:Monomer. Interacts with CSPG4 and FGFBP1. Found in a complex with FGFBP1, FGF1 and FGF2.,tissue specificity:Expressed in granulosa and cumulus cells.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
