BD-PT5542-C4a/b Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

C4a/b Rabbit Polyclonal Antibody

产品详情相关文献产品问答相关产品

产品基本信息

产品货号

BD-PT5542

产品名称

C4a/b Rabbit Polyclonal Antibody

别名

C4A; CO4; CPAMD2; Complement C4-A; Acidic complement C4; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2; C4B; CO4; CPAMD3; Complement C4-B; Basic complement C4; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3

类别

常规抗体

基因名称

C4A/C4B

蛋白名称

Complement C4-A/Complement C4-B

推荐应用

反应种属

Human,Rat,Mouse

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

720/721

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=720/721

Human Swissprot No.

P0C0L4/P0C0L5

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P0C0L4/P0C0L5/entry

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=

Rat Swissprot Link

http://www.uniprot.org/uniprot/

免疫原

The antiserum was produced against synthesized peptide derived from the Internal region of human C4A/C4B. AA range:721-770

特异性

C4a/b Polyclonal Antibody detects endogenous levels of C4a/b protein.

稀释度

WB 1:500 - 1:2000. IHC-p: 1:100-1:300. ELISA: 1:10000.. IF 1:50-200

预测分子量

180kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

This gene encodes the acidic form of complement factor 4, part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain is cleaved to release C4 anaphylatoxin, an antimicrobial peptide and a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus and type I diabetes mellitus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist, such that individuals may have 1, 2, or 3 copies of this gene. Two transcript variants encoding different isoforms have been found for this gene. [provided

组织表达

Complement component C4 is expressed at highest levels in the liver, at moderate levels in the adrenal cortex, adrenal medulla, thyroid gland, and the kidney, and at lowest levels in the heart, ovary, small intestine, thymus, pancreas and spleen. The extra-hepatic sites of expression may be important for the local protection and inflammatory response.

细胞定位

Secreted. Cell junction, synapse . Cell projection, axon . Cell projection, dendrite .

信号通路

Complement and coagulation cascades;Systemic lupus erythematosus;

功能

disease:Defects in C4A are the cause of C4A deficiency [MIM:120810].,disease:The C4A6 allotype is totally deficient in hemolytic activity.,function:C4 plays a central role in the activation of the classical pathway of the complement system. It is processed by activated C1 which removes from the alpha chain the C4a anaphylatoxin. The remaining alpha chain fragment C4b is the major activation product and is an essential subunit of the C3 convertase (C4b2a) and the C5 convertase (C3bC4b2a) enzymes of the classical complement pathway.,function:Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.,miscellaneous:C4A allotypes react more rapidly with the amino group of peptide antigens while C4B allotypes react more rapidly with the hydroxyl group of carbohydrate antigens.,online information:Blood group antigen gene mutation database,polymorphism:Human complement component C4 is polymorphic at two loci, C4A and C4B. 13 alleles of C4A and 22 alleles of C4B have been detected. The allele shown here is C4A4. The C4A alleles carry the Rodgers (Rg) while the C4B alleles carry the Chido (Ch) blood group antigens.,PTM:Prior to secretion, the single-chain precursor is enzymatically cleaved to yield the non-identical chains (alpha, beta and gamma). During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase.,similarity:Contains 1 anaphylatoxin-like domain.,similarity:Contains 1 NTR domain.,subunit:Circulates in blood as a disulfide-linked trimer of an alpha, beta and gamma chain.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western Blot analysis of AD293 cells using C4a/b Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100

Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100

Immunohistochemical analysis of paraffin-embedded Human Liver. 1, Antibody was diluted at 1:100（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.

Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:100（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.