产品名称
SPARC Rabbit Polyclonal Antibody
别名
SPARC; ON; SPARC; Basement-membrane protein 40; BM-40; Osteonectin; ON; Secreted protein acidic and rich in cysteine
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=6678
Human Swissprot No.
P09486
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P09486/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=20692
Mouse Swissprot No.
P07214
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P07214
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=24791
Rat Swissprot Link
http://www.uniprot.org/uniprot/P16975
免疫原
The antiserum was produced against synthesized peptide derived from the Internal region of human SPARC. AA range:141-190
特异性
SPARC Polyclonal Antibody detects endogenous levels of SPARC protein.
稀释度
WB 1:500 - 1:2000. IHC-p: 1:100-1:300. ELISA: 1:20000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2015],
组织表达
Muscle,Placenta,PNS,Prostate,Skin,
细胞定位
Secreted, extracellular space, extracellular matrix, basement membrane . In or around the basement membrane.
功能
developmental stage:Expressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair.,function:Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.,online information:Osteonectin entry,similarity:Belongs to the SPARC family.,similarity:Contains 1 EF-hand domain.,similarity:Contains 1 follistatin-like domain.,similarity:Contains 1 Kazal-like domain.,subcellular location:In or around the basement membrane.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
