BD-PT5490-CRF-RI Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

CRF-RI Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT5490

产品名称

CRF-RI Rabbit Polyclonal Antibody

别名

CRHR1; CRFR; CRFR1; CRHR; Corticotropin-releasing factor receptor 1; CRF-R-1; CRF-R1; CRFR-1; Corticotropin-releasing hormone receptor 1; CRH-R-1; CRH-R1

类别

常规抗体

基因名称

CRHR1

蛋白名称

Corticotropin-releasing factor receptor 1

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

1394

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1394

Human Swissprot No.

P34998

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P34998/entry

Mouse Gene ID

12921

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=12921

Mouse Swissprot No.

P35347

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P35347

Rat Gene ID

58959

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=58959

Rat Swissprot No.

P35353

Rat Swissprot Link

http://www.uniprot.org/uniprot/P35353

免疫原

The antiserum was produced against synthesized peptide derived from the Internal region of human CRHR1. AA range:71-120

特异性

CRF-RI Polyclonal Antibody detects endogenous levels of CRF-RI protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

预测分子量

50kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

This gene encodes a G-protein coupled receptor that binds neuropeptides of the corticotropin releasing hormone family that are major regulators of the hypothalamic-pituitary-adrenal pathway. The encoded protein is essential for the activation of signal transduction pathways that regulate diverse physiological processes including stress, reproduction, immune response and obesity. Alternative splicing results in multiple transcript variants. Naturally-occurring readthrough transcription between this gene and upstream GeneID:147081 results in transcripts that encode isoforms that share similarity with the products of this gene. [provided by RefSeq, Aug 2016],

组织表达

Predominantly expressed in the cerebellum, pituitary, cerebral cortex and olfactory lobe.

细胞定位

Cell membrane; Multi-pass membrane protein. Endosome. Agonist-binding promotes endocytosis.

信号通路

Neuroactive ligand-receptor interaction;Long-term depression;

功能

function:This is a receptor for corticotropin releasing factor. Shows high-affinity CRF binding. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.,PTM:C-terminal Ser or Thr residues may be phosphorylated.,similarity:Belongs to the G-protein coupled receptor 2 family.,tissue specificity:Predominantly expressed in the cerebellum, pituitary, cerebral cortex and olfactory lobe.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western Blot analysis of PC12 cells using CRF-RI Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100

Immunohistochemical analysis of paraffin-embedded mouse-brain, antibody was diluted at 1:100

Immunohistochemical analysis of paraffin-embedded Human Liver. 1, Antibody was diluted at 1:100（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.

Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:100（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.