产品名称
ECA39 Rabbit Polyclonal Antibody
别名
BCAT1; BCT1; ECA39; Branched-chain-amino-acid aminotransferase, cytosolic; BCAT(c); Protein ECA39
蛋白名称
Branched-chain-amino-acid aminotransferase, cytosolic
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=586
Human Swissprot No.
P54687
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P54687/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=12035
Mouse Swissprot No.
P24288
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P24288
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=29592
Rat Swissprot Link
http://www.uniprot.org/uniprot/P54690
免疫原
The antiserum was produced against synthesized peptide derived from the Internal region of human BCAT1. AA range:231-280
特异性
ECA39 Polyclonal Antibody detects endogenous levels of ECA39 protein.
稀释度
WB 1:500 - 1:2000. IHC-p: 1:100-1:300. ELISA: 1:20000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
branched chain amino acid transaminase 1(BCAT1) Homo sapiens This gene encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. As there is also a gene encoding a mitochondrial form of this enzyme, mutations in either gene may contribute to these disorders. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2010],
组织表达
During embryogenesis, expressed in the brain and kidney. Overexpressed in MYC-induced tumors such as Burkitt's lymphoma.
信号通路
Valine; leucine and isoleucine degradation;Valine; leucine and isoleucine biosynthesis;Pantothenate and CoA biosynthesis;
功能
catalytic activity:2-oxoglutaric acid + L-isoleucine = (S)-3-methyl-2-oxopentanoic acid + L-glutamic acid.,catalytic activity:2-oxoglutaric acid + L-valine = 3-methyl-2-oxobutanoic acid + L-glutamic acid.,catalytic activity:L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate.,cofactor:Pyridoxal phosphate.,function:Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine.,similarity:Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.,subunit:Homodimer.,tissue specificity:During embryogenesis, expressed in the brain and kidney. Overexpressed in C-myc induced tumors such as Burkitt's lymphoma.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
