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MYH-pan Rabbit Polyclonal Antibody

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产品基本信息

产品货号
BD-PT5427
产品名称
MYH-pan Rabbit Polyclonal Antibody
别名
MYH1; Myosin-1; Myosin heavy chain 1; Myosin heavy chain 2x; MyHC-2x; Myosin heavy chain IIx/d; MyHC-IIx/d; Myosin heavy chain, skeletal muscle, adult 1; MYH2; MYHSA2; Myosin-2; Myosin heavy chain 2Myosin heavy chain 2; Myosin heavy chain 2a; MyHC-2a; Myosin heavy chain IIa; MyHC-IIa; Myosin heavy chain, skeletal muscle, adult 2; MYH3; Myosin-3; Muscle embryonic myosin heavy chain; Myosin heavy chain 3
类别
常规抗体
基因名称
MYH1/MYH2/MYH3/PTPRJ/MYH6/MYH7/MYH8
蛋白名称
Myosin-1/Myosin-2/Myosin-3/Receptor-type tyrosine-protein phosphatase eta/Myosin-6/Myosin-7/Myosin-8
推荐应用
WB
反应种属
Human,Mouse,Rat
浓度
1 mg/ml
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene ID
4619/4620/4621/5795/4624/4625/4626
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=4619
Human Swissprot No.
P12882/Q9UKX2/P11055/Q9Y623/P13533/P12883/P13535
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P12882/entry
Mouse Gene ID
17879/17883/17884
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=17879
Rat Gene ID
24583/360543
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=24583
Rat Swissprot No.
P12847/Q29RW1
Rat Swissprot Link
http://www.uniprot.org/uniprot/P12847
免疫原
The antiserum was produced against synthesized peptide derived from human MYH-pan around the non-acetylation site of Lys1394. AA range:1351-1400
特异性
MYH-pan Polyclonal Antibody detects endogenous levels of MYH-pan protein only when acetylation at K1394.
稀释度
WB 1:500 - 1:2000. IHC-p: 1:100-1:300. ELISA: 1:20000.. IF 1:50-200
预测分子量
225kD
运输及保存条件
-20°C/1 year
宿主
Polyclonal, Rabbit,IgG
背景介绍
Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells. These isoforms show expression that is spatially and temporally regulated during development. [provided by RefSeq, Jul 2008],
组织表达
Skeletal muscle,
细胞定位
Cytoplasm, myofibril. Thick filaments of the myofibrils.
信号通路
Tight junction;Viral myocarditis;
功能
domain:The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.,function:Muscle contraction.,miscellaneous:Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).,similarity:Contains 1 IQ domain.,similarity:Contains 1 myosin head-like domain.,subcellular location:Thick filaments of the myofibrils.,subunit:Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).,
期货
现货
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western blot analysis of lysate from 4T1 cells, using MYH-pan Antibody.

Western Blot analysis of 4T1 cells using MYH-pan Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded human-muscle, antibody was diluted at 1:100

Immunohistochemical analysis of paraffin-embedded human-muscle, antibody was diluted at 1:100

Immunohistochemical analysis of paraffin-embedded mouse-muscle, antibody was diluted at 1:100

Immunohistochemical analysis of paraffin-embedded mouse-muscle, antibody was diluted at 1:100

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