BD-PT5396-CD166 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

CD166 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT5396

产品名称

CD166 Rabbit Polyclonal Antibody

别名

ALCAM; MEMD; CD166 antigen; Activated leukocyte cell adhesion molecule; CD166

类别

常规抗体

基因名称

ALCAM

蛋白名称

CD166 antigen

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

214

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=214

Human Swissprot No.

Q13740

Human Swissprot Link

http://www.uniprot.org/uniprotkb/Q13740/entry

Mouse Gene ID

11658

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=11658

Mouse Swissprot No.

Q61490

Mouse Swissprot Link

http://www.uniprot.org/uniprot/Q61490

Rat Gene ID

79559

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=79559

Rat Swissprot No.

O35112

Rat Swissprot Link

http://www.uniprot.org/uniprot/O35112

免疫原

The antiserum was produced against synthesized peptide derived from the C-terminal region of human ALCAM. AA range:481-530

特异性

CD166 Polyclonal Antibody detects endogenous levels of CD166 protein.

稀释度

IF 1:50-200 WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.

预测分子量

65kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Aug 2011],

组织表达

Detected on hematopoietic stem cells derived from umbilical cord blood (PubMed:24740813). Detected on lymph vessel endothelial cells, skin and tonsil (PubMed:23169771). Detected on peripheral blood monocytes (PubMed:15048703). Detected on monocyte-derived dendritic cells (at protein level) (PubMed:16352806). Detected at low levels in spleen, placenta, liver (PubMed:9502422). Expressed by activated T-cells, B-cells, monocytes and thymic epithelial cells (PubMed:7760007). Isoform 1 and isoform 3 are detected in vein and artery endothelial cells, astrocytes, keratinocytes and artery smooth muscle cells (PubMed:15496415). Expressed by neurons in the brain. Restricted expression in tumor cell lines. Detected in highly metastasizing melanoma cell lines (PubMed:9502422).

细胞定位

Cell membrane ; Single-pass type I membrane protein . Cell projection, axon . Cell projection, dendrite . Detected at the immunological synapse, i.e, at the contact zone between antigen-presenting dendritic cells and T-cells (PubMed:15294938, PubMed:16352806). Colocalizes with CD6 and the TCR/CD3 complex at the immunological synapse (PubMed:15294938). .; [Isoform 3]: Secreted .

信号通路

Cell adhesion molecules (CAMs);

功能

domain:The CD6 binding site is located in the N-terminal Ig-like domain.,function:Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system.,similarity:Contains 2 Ig-like V-type (immunoglobulin-like) domains.,similarity:Contains 3 Ig-like C2-type (immunoglobulin-like) domains.,tissue specificity:Spleen, placenta, liver, and weakly in liver. Expressed by activated T-cells, B-cells, monocytes and thymic epithelial cells. Expressed by neurons in the brain. Restricted expression in tumor cell lines. Preferentially expressed in highly metastasizing melanoma cell lines.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western blot analysis of lysate from K562 cells, using ALCAM Antibody.

Western Blot analysis of K562 cells using CD166 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100

Immunohistochemical analysis of paraffin-embedded Human Liver. 1, Antibody was diluted at 1:200（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.

Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:100（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.