BD-PT5394-CD81 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

CD81 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT5394

产品名称

CD81 Rabbit Polyclonal Antibody

别名

CD81; TAPA1; TSPAN28; CD81 antigen; 26 kDa cell surface protein TAPA-1; Target of the antiproliferative antibody 1; Tetraspanin-28; Tspan-28; CD81

类别

常规抗体

基因名称

CD81

蛋白名称

CD81 antigen

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

975

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=975

Human Swissprot No.

P60033

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P60033/entry

Mouse Gene ID

12520

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=12520

Mouse Swissprot No.

P35762

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P35762

Rat Gene ID

25621

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25621

Rat Swissprot No.

Q62745

Rat Swissprot Link

http://www.uniprot.org/uniprot/Q62745

免疫原

The antiserum was produced against synthesized peptide derived from the Internal region of human CD81. AA range:111-160

特异性

CD81 Polyclonal Antibody detects endogenous levels of CD81 protein.

稀释度

IF 1:50-200 WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.

预测分子量

26kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014],

组织表达

Expressed on B cells (at protein level) (PubMed:20237408). Expressed in hepatocytes (at protein level) (PubMed:12483205). Expressed in monocytes/macrophages (at protein level) (PubMed:12796480). Expressed on both naive and memory CD4-positive T cells (at protein level) (PubMed:22307619).

细胞定位

Cell membrane ; Multi-pass membrane protein . Basolateral cell membrane ; Multi-pass membrane protein . Associates with CLDN1 and the CLDN1-CD81 complex localizes to the basolateral cell membrane. .

信号通路

B_Cell_Antigen;

功能

function:May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May acts a the viral receptor for HCV.,PTM:Not glycosylated.,similarity:Belongs to the tetraspanin (TM4SF) family.,subunit:Plays a critical role in HCV attachment and/or cell entry by interacting with HCV E1/E2 glycoproteins heterodimer. Interacts directly with IGSF8.,tissue specificity:Hematolymphoid, neuroectodermal and mesenchymal tumor cell lines.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western blot analysis of lysate from 4T1 cells, using CD81 Antibody.

Immunofluorescence analysis of mouse-kidney tissue. 1,CD81 Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of rat-spleen tissue. 1,CD81 Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Western Blot analysis of 4T1 cells using CD81 Polyclonal Antibody. Antibody was diluted at 1:2000. Secondary antibody was diluted at 1:20000

Western blot analysis of lysates from HT-29, NIH/3T3, and HepG2 cells, primary antibody was diluted at 1:1000, 4° over night, secondary antibody was diluted at 1:10000, 37° 1hour.