BD-PT5378

Apaf-1 Rabbit Polyclonal Antibody

APAF1; KIAA0413; Apoptotic protease-activating factor 1; APAF-1

常规抗体

APAF1

Apoptotic protease-activating factor 1

WB

Human,Mouse,Rat

1 mg/ml

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

317

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=317

O14727

http://www.uniprot.org/uniprotkb/O14727/entry

11783

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=11783

O88879

http://www.uniprot.org/uniprot/O88879

78963

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=78963

Q9EPV5

http://www.uniprot.org/uniprot/Q9EPV5

The antiserum was produced against synthesized peptide derived from the Internal region of human APAF1. AA range:501-550

Apaf-1 Polyclonal Antibody detects endogenous levels of Apaf-1 protein.

WB 1:500 - 1:2000. IHC-p: 1:100-1:300. ELISA: 1:20000.. IF 1:50-200

135kD

-20°C/1 year

Polyclonal, Rabbit,IgG

This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],

Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.

Cytoplasm .

p53;Apoptosis_Inhibition;Apoptosis_Mitochondrial;Apoptosis_Overview;Alzheimer's disease;Parkinson's disease;Amyotrophic lateral sclerosis (ALS);Huntington's disease;Small cell lung cancer;

domain:The CARD domain mediates interaction with APIP.,function:Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.,induction:By E2F and p53 in apoptotic neurons.,similarity:Contains 1 CARD domain.,similarity:Contains 1 NB-ARC domain.,similarity:Contains 13 WD repeats.,subunit:Monomer. Oligomerizes upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP.,tissue specificity:Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.,

现货

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.