BD-PT5362-Troponin T-C Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Troponin T-C Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT5362

产品名称

Troponin T-C Rabbit Polyclonal Antibody

别名

TNNT2; Troponin T, cardiac muscle; TnTc; Cardiac muscle troponin T; cTnT

类别

常规抗体

基因名称

TNNT2

蛋白名称

Troponin T cardiac muscle

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

7139

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=7139

Human Swissprot No.

P45379

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P45379/entry

Mouse Gene ID

21956

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=21956

Mouse Swissprot No.

P50752

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P50752

Rat Gene ID

24837

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=24837

Rat Swissprot No.

P50753

Rat Swissprot Link

http://www.uniprot.org/uniprot/P50753

免疫原

The antiserum was produced against synthesized peptide derived from the Internal region of human TNNT2. AA range:131-180

特异性

Troponin T-C Polyclonal Antibody detects endogenous levels of Troponin T-C protein.

稀释度

WB 1:500 - 1:2000. IHC-p: 1:100-1:300. ELISA: 1:20000.. IF 1:50-200

预测分子量

35kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq, Jul 2008],

组织表达

Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.

细胞定位

cytosol,troponin complex,striated muscle thin filament,sarcomere,

信号通路

Cardiac muscle contraction;Hypertrophic cardiomyopathy (HCM);Dilated cardiomyopathy;

功能

alternative products:Additional isoforms seem to exist. Experimental confirmation may be lacking for some isoforms,disease:Defects in TNNT2 are the cause of cardiomyopathy dilated type 1D (CMD1D) [MIM:601494]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.,disease:Defects in TNNT2 are the cause of cardiomyopathy familial hypertrophic type 2 (CMH2) [MIM:115195]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.,disease:Defects in TNNT2 are the cause of cardiomyopathy familial restrictive type 3 (RCM3) [MIM:612422]. Restrictive cardiomyopathy is a heart disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.,function:Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.,similarity:Belongs to the troponin T family.,tissue specificity:Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western blot analysis of lysate from AD293, 4T1 cells, using TNNT2 Antibody.

Western Blot analysis of AD293, 4T1 cells using Troponin T-C Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded mouse-heart, antibody was diluted at 1:100

Immunohistochemical analysis of paraffin-embedded Human liver. 1, Antibody was diluted at 1:200（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.

Immunohistochemical analysis of paraffin-embedded Human heart. 1, Antibody was diluted at 1:200（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.