BD-PT5351-MPO Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

MPO Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT5351

产品名称

MPO Rabbit Polyclonal Antibody

别名

MPO; Myeloperoxidase; MPO

类别

常规抗体

基因名称

MPO

蛋白名称

Myeloperoxidase

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

4353

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=4353

Human Swissprot No.

P05164

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P05164/entry

Mouse Gene ID

17523

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=17523

Mouse Swissprot No.

P11247

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P11247

免疫原

The antiserum was produced against synthesized peptide derived from the N-terminal region of human MPO. AA range:41-90

特异性

MPO Polyclonal Antibody detects endogenous levels of MPO protein.

稀释度

IHC-p: 100-300.WB 1:500 - 1:2000. ELISA: 1:20000.. IF 1:50-200

预测分子量

85kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. [provided by RefSeq, Nov 2014],

组织表达

Leukemia,Leukocyte,Liver,Plasma,Saliva,

细胞定位

Lysosome.

功能

catalytic activity:Cl(-) + H(2)O(2) = HOCl + 2 H(2)O.,catalytic activity:Donor + H(2)O(2) = oxidized donor + 2 H(2)O.,cofactor:Binds 1 calcium ion per heterodimer.,cofactor:Binds 1 heme B (iron-protoporphyrin IX) group covalently per heterodimer.,disease:Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.,function:Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.,online information:MPO mutation db,online information:Myeloperoxidase entry,similarity:Belongs to the peroxidase family. XPO subfamily.,subunit:Tetramer of two light chains and two heavy chains.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western blot analysis of lysate from K562 cells, using MPO Antibody.

Western Blot analysis of K562 cells using MPO Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded Human lung. 1, Antibody was diluted at 1:100（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.

Immunohistochemical analysis of paraffin-embedded Human lymphoma. 1, Antibody was diluted at 1:100（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.