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EPAS-1 Rabbit Polyclonal Antibody

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产品基本信息

产品货号
BD-PT5325
产品名称
EPAS-1 Rabbit Polyclonal Antibody
别名
EPAS1; BHLHE73; HIF2A; MOP2; PASD2; Endothelial PAS domain-containing protein 1; EPAS-1; Basic-helix-loop-helix-PAS protein MOP2; Class E basic helix-loop-helix protein 73; bHLHe73;HIF-1-alpha-like factor; HLF; Hypoxia-inducible factor 2-alpha; HIF-2-alpha; HIF2-alpha; Member of PAS protein 2; PAS domain-containing protein 2
类别
常规抗体
基因名称
EPAS1 BHLHE73 HIF2A MOP2 PASD2
蛋白名称
Endothelial PAS domain-containing protein 1
推荐应用
WB
反应种属
Human,Mouse,Rat
浓度
1 mg/ml
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene ID
2034
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=2034
Human Swissprot No.
Q99814
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q99814/entry
Mouse Gene ID
13819
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=13819
Mouse Swissprot No.
P97481
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P97481
Rat Gene ID
29452
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=29452
Rat Swissprot No.
Q9JHS1
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q9JHS1
免疫原
Synthesized peptide derived from human EPAS-1 around the non-acetylation site of K385.
特异性
EPAS-1 Polyclonal Antibody detects endogenous levels of EPAS-1 protein.
稀释度
IF 1:50-200 WB 1:500 - 1:2000. IHC-p: 1:100-1:300. ELISA: 1:20000. Not yet tested in other applications.
参考分子量
96kD
预测分子量
110-120kD
运输及保存条件
-20°C/1 year
宿主
Polyclonal, Rabbit,IgG
背景介绍
endothelial PAS domain protein 1(EPAS1) Homo sapiens This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq, Nov 2009],
组织表达
Expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells.
细胞定位
Nucleus . Nucleus speckle . Colocalizes with HIF3A in the nucleus and speckles. .
信号通路
Pathways in cancer;Renal cell carcinoma;
功能
disease:Defects in EPAS1 are the cause of erythrocytosis familial type 4 (ECYT4) [MIM:611783]. ECYT4 is an autosomal dominant disorder characterized by increased serum red blood cell mass, elevated hemoglobin concentration and hematocrit, and normal platelet and leukocyte counts.,function:Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD.,PTM:In normoxia, is hydroxylated on Asn-847 by HIF1AN thus probably abrogating interaction with CREBBP and EP300 and preventing transcriptional activation.,PTM:In normoxia, is probably hydroxylated on Pro-405 and Pro-531 by EGLN1/PHD1, EGLN2/PHD2 and/or EGLN3/PHD3. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization.,PTM:Phosphorylated on multiple sites in the CTAD.,PTM:The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,similarity:Contains 1 PAC (PAS-associated C-terminal) domain.,similarity:Contains 2 PAS (PER-ARNT-SIM) domains.,subunit:Efficient DNA binding requires dimerization with another bHLH protein. Heterodimerizes with ARNT. Interacts with CREBBP.,tissue specificity:Expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells.,
期货
现货
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of human-stomach tissue. 1,EPAS-1 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of human-stomach tissue. 1,EPAS-1 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of mouse-spleen tissue. 1,EPAS-1 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of mouse-spleen tissue. 1,EPAS-1 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,EPAS-1 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1,EPAS-1 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,EPAS-1 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1,EPAS-1 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,EPAS-1 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,EPAS-1 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

Western Blot analysis of Jurkat, L929 cells using EPAS-1 Polyclonal Antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded human-mammary-cancer, antibody was diluted at 1:100

Immunohistochemical analysis of paraffin-embedded human-mammary-cancer, antibody was diluted at 1:100

Immunohistochemical analysis of paraffin-embedded human-mammary-cancer, antibody was diluted at 1:100

Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:100

Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:100

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