产品名称
IDH3A Rabbit Polyclonal Antibody
别名
IDH3A; Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial; Isocitric dehydrogenase subunit alpha; NAD(+)-specific ICDH subunit alpha
蛋白名称
Isocitrate dehydrogenase [NAD] subunit alpha mitochondrial
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=3419
Human Swissprot No.
P50213
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P50213/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=67834
Mouse Swissprot No.
Q9D6R2
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9D6R2
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=114096
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q99NA5
免疫原
Synthesized peptide derived from the Internal region of human IDH3A.
特异性
IDH3A Polyclonal Antibody detects endogenous levels of IDH3A protein.
稀释度
WB 1:500 - 1:2000. IHC-p: 1:100-1:300. ELISA: 1:20000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. [provided by RefSeq, Jul 2008],
组织表达
Brain,Brain cortex,Cajal-Retzius cell,Esophagus tumor,Fetal
信号通路
Citrate cycle (TCA cycle);
功能
catalytic activity:Isocitrate + NAD(+) = 2-oxoglutarate + CO(2) + NADH.,cofactor:Binds 1 magnesium or manganese ion per subunit.,similarity:Belongs to the isocitrate and isopropylmalate dehydrogenases family.,subunit:Heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
