BD-PT5297-VEGF-C Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

VEGF-C Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT5297

产品名称

VEGF-C Rabbit Polyclonal Antibody

别名

VEGFC; Vascular endothelial growth factor C; VEGF-C; Flt4 ligand; Flt4-L; Vascular endothelial growth factor-related protein; VRP

类别

常规抗体

基因名称

VEGFC

蛋白名称

Vascular endothelial growth factor C

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

7424

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=7424

Human Swissprot No.

P49767

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P49767/entry

Mouse Gene ID

22341

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=22341

Mouse Swissprot No.

P97953

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P97953

Rat Gene ID

114111

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=114111

Rat Swissprot No.

O35757

Rat Swissprot Link

http://www.uniprot.org/uniprot/O35757

免疫原

The antiserum was produced against synthesized peptide derived from the Internal region of human VEGFC. AA range:91-140

特异性

VEGF-C Polyclonal Antibody detects endogenous levels of VEGF-C protein.

稀释度

WB 1:500 - 1:2000. IHC-p: 1:100-1:300. ELISA: 1:20000.. IF 1:50-200

预测分子量

42kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family. The encoded protein promotes angiogenesis and endothelial cell growth, and can also affect the permeability of blood vessels. The proprotein is further cleaved into a fully processed form that can bind and activate VEGFR-2 and VEGFR-3 receptors. [provided by RefSeq, Apr 2014],

组织表达

Spleen, lymph node, thymus, appendix, bone marrow, heart, placenta, ovary, skeletal muscle, prostate, testis, colon and small intestine and fetal liver, lung and kidney, but not in peripheral blood lymphocyte.

细胞定位

Secreted.

信号通路

Cytokine-cytokine receptor interaction;mTOR;Focal adhesion;Pathways in cancer;Renal cell carcinoma;Pancreatic cancer;Bladder cancer;

功能

function:Growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (Flk1) and VEGFR-3 (Flt4) receptors.,PTM:Undergoes a complex proteolytic maturation which generates a variety of processed secreted forms with increased activity toward VEGFR-3, but only the fully processed form could activate VEGFR-2. VEGF-C first form an antiparallel homodimer linked by disulfide bonds. Before secretion, a cleavage occurs between Arg-227 and Ser-228 producing an heterotetramer. The next extracellular step of the processing removes the N-terminal propeptide. Finally the mature VEGF-C is composed mostly of two VEGF homology domains (VHDs) bound by non-covalent interactions.,similarity:Belongs to the PDGF/VEGF growth factor family.,subunit:Homodimer; non-covalent and antiparallel.,tissue specificity:Spleen, lymph node, thymus, appendix, bone marrow, heart, placenta, ovary, skeletal muscle, prostate, testis, colon and small intestine and fetal liver, lung and kidney, but not in peripheral blood lymphocyte.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western blot analysis of lysate from L929 cells, using VEGFC Antibody.

Western Blot analysis of L929 cells using VEGF-C Polyclonal Antibody. Antibody was diluted at 1:2000. Secondary antibody was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded mouse-heart, antibody was diluted at 1:100

Immunohistochemical analysis of paraffin-embedded Human Liver. 1, Antibody was diluted at 1:200（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.

Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:200（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.