BD-PT5218

Keap1 Rabbit Polyclonal Antibody

KEAP1; INRF2; KIAA0132; KLHL19; Kelch-like ECH-associated protein 1; Cytosolic inhibitor of Nrf2; INrf2; Kelch-like protein 19

常规抗体

KEAP1

Kelch-like ECH-associated protein 1

WB

Human,Mouse,Rat

1 mg/ml

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

9817

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=9817

Q14145

http://www.uniprot.org/uniprotkb/Q14145/entry

50868

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=50868

Q9Z2X8

http://www.uniprot.org/uniprot/Q9Z2X8

117519

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=117519

P57790

http://www.uniprot.org/uniprot/P57790

The antiserum was produced against synthesized peptide derived from the Internal region of human KEAP1. AA range:411-460

Keap1 Polyclonal Antibody detects endogenous levels of Keap1 protein.

WB 1:500 - 1:2000. IHC-p: 1:100-300 ELISA: 1:20000.. IF 1:50-200

70kD

-20°C/1 year

Polyclonal, Rabbit,IgG

This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq, Jul 2008],

Broadly expressed, with highest levels in skeletal muscle.

Cytoplasm . Nucleus . Mainly cytoplasmic (PubMed:15601839). In response to selective autophagy, relocalizes to inclusion bodies following interaction with SQSTM1/p62 (PubMed:20452972). .

Ubiquitin mediated proteolysis;

disease:Defects in KEAP1 may be a cause of breast cancer.,disease:Defects in KEAP1 may be involved in non small cell lung carcinomas (NSCLC) and lung adenocarcinoma.,domain:The Kelch repeats mediate interaction with NF2L2/NRF2, BPTF and PGAM5.,enzyme regulation:Ubiquitination and subsequent degradation of PGAM5 is inhibited by oxidative stress and sulforaphane.,function:Retains NFE2L2/NRF2 in the cytosol. Functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. May also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome.,PTM:Ubiquitinated and subject to proteasomal degradation.,similarity:Contains 1 BACK (BTB/Kelch associated) domain.,similarity:Contains 1 BTB (POZ) domain.,similarity:Contains 6 Kelch repeats.,subcellular location:Shuttles between cytoplasm and nucleus.,subunit:Homodimer. Interacts with the N-terminal regulatory domain of NF2L2/NRF2. Interacts with BPTF and PTMA. Interacts with CUL3. Part of a complex that contains KEAP1, CUL3 and RBX1. Interacts with PGAM5.,tissue specificity:Broadly expressed, with highest levels in skeletal muscle.,

现货

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.