产品名称
Cleaved PARP(Mix)Mouse Monoclonal Antibody
别名
PARP1; ADPRT; PPOL; Poly [ADP-ribose] polymerase 1; PARP-1; ADP-ribosyltransferase diphtheria toxin-like 1; ARTD1; NAD(+) ADP-ribosyltransferase 1; ADPRT 1; Poly[ADP-ribose] synthase 1
蛋白名称
Poly [ADP-ribose] polymerase 1
存储缓冲液
PBS, pH 7.4, containing 0.5%BSA, 0.02% New type preservative N as Preservative and 50% Glycerol.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=142
Human Swissprot No.
P09874
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P09874/entry
Mouse Swissprot No.
P11103
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P11103
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25591
Rat Swissprot Link
http://www.uniprot.org/uniprot/P27008
免疫原
Synthetic Peptide of Cleaved PARP
特异性
The antibody detects endogenous pro and active PARP protein.
稀释度
IF 1:50-200 WB 1:2000-5000 IHC 1:50-300
背景介绍
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008],
组织表达
Brain,Colon carcinoma,Fibroblast,Lung,Ovarian carcinoma,Skin,
细胞定位
Nucleus . Nucleus, nucleolus . Chromosome . Localizes to sites of DNA damage. .
信号通路
Base excision repair;
功能
catalytic activity:NAD(+) + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.,function:Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.,miscellaneous:The ADP-D-ribosyl group of NAD(+) is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units.,PTM:Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.,PTM:Poly-ADP-ribosylated by PARP2.,similarity:Contains 1 BRCT domain.,similarity:Contains 1 PARP alpha-helical domain.,similarity:Contains 1 PARP catalytic domain.,similarity:Contains 2 PARP-type zinc fingers.,subunit:Component of a base excision repair (BER) complex, containing at least XRCC1, PARP2, POLB and LIG3. Homo- and heterodimer with PARP2. Interacts with PARP3, APTX and SRY. The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70. Interacts with TIAM2 and ZNF423.,
纯化
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
